Background: The aim of the present study was to assess the reliability of a new strategy for monitoring the serological response against Bovine Herpesvirus 1 (BoHV1), the causative agent of infectious bovine rhinotracheitis (IBR). Bulk milk samples have already been identified as cost effective diagnostic matrices for monitoring purposes. Nevertheless, most eradication programs are still based on individual standard assays. In a region of northwestern Italy (Piedmont), the voluntary eradication program for IBR has become economically unsustainable. Being the prevalence of infection still high, glycoprotein E-deleted marker vaccines are commonly used but gE blocking ELISAs are less sensitive on bulk milk samples compared to blood serum.
Results: A recently developed indirect gE ELISA showed high versatility when applied to a wide range of matrices. In this study, we applied a faster, cost effective system for the concentration of IgG from pooled milk samples. The IgG enriched fractions were tested using a gE indirect ELISA for monitoring purposes in IBR-positive and IBR-marker-vaccinated herds. Official diagnostic tests were used as gold standard. During a 3 years study, a total 250 herds were involved, including more than 34,500 lactating cows. The proposed method showed a very good agreement with official diagnostic protocols and very good diagnostic performances: only 37 positive animals were not detected across the entire study.
Conclusions: The results highlighted the ability of the proposed method to support the surveillance of IBR in the Piedmont region, reducing the costs without affecting the diagnostic performances.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282388 | PMC |
| http://dx.doi.org/10.1186/s12917-018-1716-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Use of approved cefazolin based intramammary dry goat treatment and its effects on the milk microbiota.
Sci Rep
January 2025
Department of Veterinary Medicine and Animal Sciences (DIVAS), Università degli Studi di Milano, Lodi, 26900, Italy.
Intramammary dry-off treatment is widely considered an effective method for preventing and curing intramammary infection (IMI) in lactating cows; however, it is not commonly used in small ruminants like goats. Therefore, this study was designed to evaluate the effect of an approved cefazolin-based intramammary treatment on the milk microbiota of Alpine dairy goats during the dry and early lactation periods. Sixty goats were randomly selected based on bacteriological results and randomly allocated into the control group (CG) or the treatment group (TG).
View Article and Find Full Text PDFFermented foods affect the seasonal stability of gut bacteria in an Indian rural population.
Nat Commun
January 2025
Laboratory of Microbiology, Wageningen University & Research, 6708, WE, Wageningen, The Netherlands.
The effect of fermented foods on healthy human gut microbiota structure and function, particularly its seasonal preference and frequent long-term consumption, has been largely uncharacterised. Here, we assess the gut microbiota and metabolite composition of 78 healthy Indian agrarian individuals who differ in the intake of fermented milk and soybean products by seasonal sampling during hot-humid summer, autumn and dry winter. Here we show that, seasonal shifts between the Prevotella- and Bifidobacterium/Ruminococcus-driven community types, or ecological states, and associated fatty acid derivatives, with a bimodal change in Bacteroidota community structure during summer, particularly in fermented milk consumers.
View Article and Find Full Text PDFIntermittent and increasing intravenous lipopolysaccharide's impact on metabolism, inflammation and production in lactating dairy cows.
J Dairy Sci
January 2025
Department of Animal Science, Iowa State University, Ames, Iowa 50011. Electronic address:
Experimental objectives were to create a chronic inflammatory model to evaluate the effects of persistent immune activation on metabolism, inflammation, and productivity in lactating dairy cows. Twelve lactating Holstein cows (631 ± 16 kg BW; 124 ± 15 DIM) were enrolled in a study with 2 experimental periods (P); during P1 (5 d), cows were fed ad libitum and baseline data were obtained. At the initiation of P2 (7 d), cows were assigned to 1 of 2 treatments: 1) saline-infused and pair-fed (PF; 5 mL intravenously (IV) sterile saline on d 1, 3, and 5; n = 6) or 2) lipopolysaccharide infused and ad libitum-fed (LPS; 0.
View Article and Find Full Text PDFCharacterization of Volatile Compounds in Mozzarella Cheeses Made from Bovine and Buffalo Milk by SPME-GC×GC-O-TOFMS, SPME-Arrow-GC-MS and GC-IMS.
J Dairy Sci
January 2025
Key Laboratory of Geriatric Nutrition and Health (Beijing Technology and Business University), Ministry of Education, Beijing 100048, China. Electronic address:
High moisture Mozzarella cheese is particularly popular because of its freshness and milky flavor, however the difference in aroma compound composition between high moisture Mozzarella cheese made from bovine (BOC) and buffalo milk (BUC) remains unclear. Herein, the volatile compounds of 2 kinds of Mozzarella cheese were qualitatively and quantitatively analyzed by SPME-GC × GC-O-TOFMS, SPME-Arrow-GC-MS and GC-IMS for the first time. A total of 139 volatile compounds were identified (69 aroma active compounds were sniffed), of which 106 were identified in BOC and 96 in BUC.
View Article and Find Full Text PDFEffect of bovine and buffalo ghee fractionation on triacylglycerols profile, lipids nutritional quality indices, thermal behavior, and tocopherols content: A comparative analysis with two categories of infant formula fat.
J Dairy Sci
January 2025
Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University (UAEU), Al Ain, 15551, United Arab Emirates. Electronic address:
The increased use of dairy fat in various applications is facilitated by its fractionation into hard and liquid fractions. Herein, the fractionation of bovine ghee and buffalo ghee was investigated, and triacylglycerol (TAG) profiles of milk fat fractions and 2 categories of infant formulas fat were quantified by using ultra-performance convergence chromatography quadrupole time-of-flight mass spectrometry (UPC-Q-TOF-MS) using carbon dioxide as the mobile phase. Furthermore, the thermal behavior of the different samples was evaluated, and tocopherols content was also quantified.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!