Genetic Heterogeneity of Strains Revealed by RFLP Analysis of Region and Gene.

PCR-RFLP targeting of the 16S rDNA and genes, as well as the region, was applied to identify and differentiate between the spoilage and non-spoilage species. Eight reference strains and 75 strains isolated from spoiled juices, juice concentrates, drinks, its intermediates, and fresh apples were subject to study. Hin6I restriction patterns of the 16S rDNA gene enabled distinguishing between all the species analyzed, while the gene and gene cluster analysis also revealed that there were two major types among the isolates, one similar to the reference strain DSM 2498, and the other similar to the reference strain ATCC 49025. Heterogeneity was also observed among the isolates. RFLP analysis of the 16S rDNA and genes, as well as region, can be used successfully in the identification and research of intraspecies heterogeneity of the species.