AI Article Synopsis
- The IGRA QuantiFERON-TB Gold Plus (QFT-Plus) has been updated to include two antigen tubes, TB1 and TB2, targeting specific T-cell responses to Mycobacterium tuberculosis.
- TB1 primarily stimulates CD4 T-cells for cell-mediated responses, while TB2 is designed to also engage CD8 T-cells by utilizing newly developed peptides.
- Research demonstrated that specific CD4 T-cell clones responded to both tubes, but CD8 T-cell clones showed a much stronger response (>140-fold difference) primarily to TB2, confirming its effectiveness in detecting CD8 T-cell activity.
Article Abstract
The new generation of the IGRA QuantiFERON-TB Gold Plus (QFT-Plus) includes two antigen tubes, TB1 and TB2 which contain specific Mycobacterium tuberculosis peptides designed to stimulate both CD4 and CD8 T-cells. TB1 is designed to target cell mediated responses from CD4 T-cells, while TB2 contains newly designed peptides designed to also stimulate CD8 T-cells. We identified specific CD4 and CD8 T-cell clones that recognize different regions spanning the length of the CFP-10 protein in M. tuberculosisto directly test in QFT-Plus TB1 and TB2 tubes, followed by Interferon-gamma detection by the QFT-Plus ELISA. These clones showed specific responses to the different QFT-Plus tubes, the CD4 T-cell clone showed dose-dependent responses to both TB1 and TB2 tubes, while the CD8 T-cell clones showed specific and targeted responses to the QFT-Plus TB2 tube (>140-fold difference) versus the QFT-Plus TB1 tubes using the QFT-Plus ELISA. This testing provides direct evidence of the specificity of CD8 T-cell mediated response in QFT-Plus TB2 tubes.
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| http://dx.doi.org/10.1016/j.tube.2018.10.014 | DOI Listing |
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