Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354267 | PMC |
| http://dx.doi.org/10.1105/tpc.18.00923 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Possible Origin of Life in Nonpolar Environments.
Biosystems
December 2024
University of Maribor, Faculty of Natural Sciences and Mathematics, Koroška cesta 160, 2000 Maribor, Slovenia; University of Maribor, Faculty of Medicine, Taborska ulica 6B, 2000 Maribor, Slovenia. Electronic address:
Explaining the emergence of life is perhaps the central and most challenging question in modern science. We are proposing a new hypothesis concerning the origins of life. The new hypothesis is based on the assumption that during the emergence of life, evolution had to first involve autocatalytic systems which only subsequently acquired the capacity of genetic heredity.
View Article and Find Full Text PDFThe influence of potassium on folding parameters of porcine kidney betaine aldehyde dehydrogenase.
Biochem Biophys Res Commun
January 2025
Departamento de Ciencias Químico-Biológicas y Agropecuarias, Universidad de Sonora, Lázaro Cárdenas 100, Colonia Francisco Villa, Navojoa, 85880, Mexico. Electronic address:
Porcine kidney betaine aldehyde dehydrogenase (pkBADH) uses NAD as a coenzyme to convert betaine aldehyde to glycine betaine. In previous studies we described the impact of potassium on the affinity of pkBADH for NAD, the effect on the tertiary and secondary structure, and changes in the flexibility of the amino acids involved in the formation of the pkBADH-NAD. However, there are still unanswered questions about how K influences the folding and maintenance of the quaternary structure.
View Article and Find Full Text PDFQuantitative dynamics of intracellular NMN by genetically encoded biosensor.
Biosens Bioelectron
January 2025
Sino-European Center of Biomedicine and Health, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 518055, Shenzhen, China. Electronic address:
Nicotinamide mononucleotide (NMN) is the direct precursor and a major booster of NAD with increasing applications in NAD- and aging-related pathologies. However, measuring live cell NMN dynamics was not possible, leaving key questions in NMN uptake and intracellular regulation unanswered. Here we developed genetically encoded bioluminescent and fluorescent sensors to quantify subcellular NMN in live cells by engineering specific NMN-responsive protein scaffolds fused to luciferase and fluorescent proteins.
View Article and Find Full Text PDFOn the production of singlet oxygen by the isoalloxazine ring in free and protein-bound flavin cofactors.
Biophys Chem
January 2025
Center for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Šafárik University in Košice, Jesenná 5, 041 54 Košice, Slovakia; Department of Biochemistry, Faculty of Science, P.J. Šafárik University in Košice, Moyzesova 11, 041 54 Košice, Slovakia. Electronic address:
Flavin cofactors, flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN), as a part of flavoenzymes play a critical role in the catalysis of multiple reactions predominantly of a redox nature. Question arises why nature developed two very similar cofactors with an identical functional part - isoalloxazine ring. We believe that an answer is related to the fact that the isoalloxazine ring belongs to endogenous photosensitizers able to produce reactive and potentially harmful singlet oxygen, O, with high efficiency, Φ ∼ 0.
View Article and Find Full Text PDFThe type 2 cytokine Fc-IL-4 revitalizes exhausted CD8 T cells against cancer.
Nature
October 2024
Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Current cancer immunotherapy predominately focuses on eliciting type 1 immune responses fighting cancer; however, long-term complete remission remains uncommon. A pivotal question arises as to whether type 2 immunity can be orchestrated alongside type 1-centric immunotherapy to achieve enduring response against cancer. Here we show that an interleukin-4 fusion protein (Fc-IL-4), a typical type 2 cytokine, directly acts on CD8 T cells and enriches functional terminally exhausted CD8 T (CD8 T) cells in the tumour.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!