cytotoxicity evaluation of thiourea derivatives bearing constituent against HK-1 cell lines.

In searching for drugs from natural product scaffolds has gained interest among researchers. In this study, a series of twelve halogenated thiourea ( chemical modification of aspirin (a natural product derivative) and evaluated for cytotoxic activity against nasopharyngeal carcinoma (NPC) cell lines, HK-1 via MTS-based colorimetric assay. The cytotoxicity studies demonstrated that halogens at position of showed promising activity against HK-1 cells (IC value ≤15 µM) in comparison to cisplatin, a positive cytotoxic drug (IC value =8.9 ± 1.9 µM). , bearing iodine at position, showed robust cytotoxicity against HK-1 cells with an IC value of 4.7 ± 0.7 µM. Molecular docking interactions between and cyclooxygenase-2 demonstrated a robust binding affinity value of -8.1 kcal/mol as compared to aspirin's binding affinity value of -6.4 kcal/mol. The findings represent a promising lead molecule from natural product with excellent cytotoxic activity against NPC cell lines.