AI Article Synopsis
- The study demonstrated that Pseudomonas mendocina CH50 can efficiently utilize triacylglycerides (TAGs) to create a new type of polyhydroxyalkanoates (mcl-PHAs) with unique properties.
- A high yield of 58% dry cell weight (dcw) was achieved using coconut oil, resulting in a terpolymer made up of three different monomer units.
- Biocompatibility tests showed that the produced mcl-PHA (P(3HO-3HD-3HDD)) is suitable for neural tissue engineering, supporting cell attachment and growth of neuronal cells.
Article Abstract
This study validated the utilization of triacylglycerides (TAGs) by Pseudomonas mendocina CH50, a wild type strain, resulting in the production of novel mcl-PHAs with unique physical properties. A PHA yield of 58% dcw was obtained using 20 g/L of coconut oil. Chemical and structural characterisation confirmed that the mcl-PHA produced was a terpolymer comprising of three different repeating monomer units, 3-hydroxyoctanoate, 3-hydroxydecanoate and 3-hydroxydodecanoate or P(3HO-3HD-3HDD). Bearing in mind the potential of P(3HO-3HD-3HDD) in biomedical research, especially in neural tissue engineering, in vitro biocompatibility studies were carried out using NG108-15 (neuronal) cells. Cell viability data confirmed that P(3HO-3HD-3HDD) supported the attachment and proliferation of NG108-15 and was therefore confirmed to be biocompatible in nature and suitable for neural regeneration.
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| http://dx.doi.org/10.1007/s10856-018-6183-9 | DOI Listing |
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