Metastasis remains a clinically unsolved issue in nasopharyngeal carcinoma. Here, we report that higher levels of cytoplasmic leukemia inhibitory factor (LIF) and LIF receptor are correlated with poorer metastasis/recurrence-free survival. Further, single nucleotide variations and signal peptide mutation of LIF are identified in NPC. Cytoplasmic LIF reprograms the invasive mode from collective to mesenchymal migration via acquisition of EMT and invadopodia-associated characteristics. Higher cytoplasmic LIF enhances cancer vascular dissemination and local invasion mechanistically through modulation of YAP1-FAK/PXN signaling. Immunohistochemical analyses of NPC biopsies reveal a positive correlation of cytoplasmic LIF expression with focal adhesion kinases. Pharmaceutical intervention with AZD0530 markedly reverses LIF-mediated cancer dissemination and local invasion through promotion of cytoplasmic accumulation of YAP1 and suppression of focal adhesion kinases. Given the significant role of LIF/YAP1-focal adhesion signaling in cancer dissemination, targeting of this pathway presents a promising opportunity to block metastasis.
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http://dx.doi.org/10.1038/s41467-018-07660-6 | DOI Listing |
Adv Sci (Weinh)
December 2024
Clinical Medicine Research Institute, Zhejiang Provincial People's Hospital, Hangzhou Medical College, 158 Shangtang Road, Hangzhou, Zhejiang, 310014, China.
Toxicol In Vitro
December 2024
College of Animal Science and Technology, Yangtze University, Jingzhou 434025, Hubei, China. Electronic address:
Chloroquine (CQ) is widely used in the therapy against malarial, tumor and recently the COVID-19 pandemic, as a lysosomotropic agent to inhibit the endolysosomal trafficking in the autophagy pathway. We previously reported that CQ (20 μM, 36 h) could reprogram transcriptome, and impair multiple signaling pathways vital to porcine immature Sertoli cells (iSCs). However, whether CQ treatment could affect the metabolomic compositions of porcine iSCs remains unclear.
View Article and Find Full Text PDFChemosphere
August 2024
Higher Institute of Arts and Crafts of Tataouine, University of Gabes, Rue OmarrEbenkhattab, 6029, Zerig-Gabes, Tunisia; Laboratory of Pastoral Ecosystems and Promotion of Spontaneous Plants and Associated Micro-organisms, Institute of Arid Land, 4100, Medenine, University of Gabes Tunisia, Tunisia. Electronic address:
Toxic heavy metal accumulation in edible plants has become a problem worth worrying about for human health. Cadmium is one of the most toxic metals presenting high bioavailability in the environment. The main route of transfer of Cd to humans is the consumption of contaminated food which suggests that reducing of Cd absorption by plants could reduce this risk.
View Article and Find Full Text PDFAnimals (Basel)
June 2024
Animal, Dairy, and Veterinary Sciences Department, Utah State University, 4815 Old Main Hill, Logan, UT 84322, USA.
Bovine in vitro oocyte maturation (IVM) is an easy way to obtain oocytes for subsequent assisted reproductive techniques but is inefficient compared to in vivo maturation. Supplementation of three cytokines, fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1), or FLI, has increased oocyte maturation and embryo development in multiple species, but studies have not explored the oocyte differences caused by FLI IVM supplementation. This study aimed to assess important nuclear and cytoplasmic maturation events in high-quality oocytes.
View Article and Find Full Text PDFNeuro Oncol
August 2024
School of pharmacy, Anhui Medical University, Hefei, China.
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