AI Article Synopsis
- The study explored using blood microRNA (miRNA) analysis as a method to detect malignant elements in human pluripotent stem cells (hPSCs) and their derivatives after transplantation.
- Researchers found that while conventional hPSC lines created mature teratomas, induced hPSCs and malignant germ cell tumors displayed undifferentiated and potentially harmful cells in mouse models.
- Key miRNAs, particularly from the miR-371 family, were linked to these malignant components and could be traced in mouse plasma, suggesting a reliable way to monitor for risks post-transplantation.
Article Abstract
Predicting developmental potency and risk of posttransplantation tumor formation by human pluripotent stem cells (hPSCs) and their derivatives largely rely on classical histological analysis of teratomas. Here, we investigated whether an assay based on microRNAs (miRNA) in blood plasma is able to detect potentially malignant elements. Several hPSCs and human malignant germ cell tumor (hGCT) lines were investigated in vitro and in vivo after mouse xenografting. The multiple conventional hPSC lines generated mature teratomas, while xenografts from induced hPSCs (hiPSCs) with reactivated reprogramming transgenes and hGCT lines contained undifferentiated and potentially malignant components. The presence of these elements was reflected in the mRNA and miRNA profiles of the xenografts with OCT3/4 mRNA and the miR-371 and miR-302 families readily detectable. miR-371 family members were also identified in mouse plasma faithfully reporting undifferentiated elements in the xenografts. This study demonstrated that undifferentiated and potentially malignant cells could be detected in vivo.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294243 | PMC |
| http://dx.doi.org/10.1016/j.stemcr.2018.11.002 | DOI Listing |
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