AI Article Synopsis
- The study investigates the role of Sec62 in cancer, showing its importance in tumor cell migration and stress tolerance.
- Researchers performed Sec62/Ki67 dual staining on 100 cervical cytology specimens to identify high-grade dysplasia and cancer.
- The results indicated that all high-grade cervical lesions (CIN3+) tested positive for the staining, demonstrating its effectiveness and reliability in detecting high-grade dysplastic lesions and assessing cancer risk.
Article Abstract
Purpose: In the previous studies, we demonstrated that Sec62 is essential for tumor cell migration, epithelial-to-mesenchymal transition, and intracellular stress tolerance. An increase in Sec62 expression correlated with an increase in cervical dysplasia severity in liquid-based cytology specimens. Ki67 is an established proliferation marker. Thus, in this study, we examined a method of Sec62/Ki67 dual staining for the detection of high-grade dysplasia and cancer in cervical liquid-based cytology specimens.
Methods: Sec62/Ki67 dual staining was performed on 100 cervical liquid-based cytology specimens. The staining results were correlated with cytological, immunocytological (p16/Ki67), colposcopic, and histological findings.
Results: All 56 (n = 56, 100%) cases of cervical intraepithelial neoplasia grade 3 and cervical cancer (CIN3+ lesions) were positive for Sec62/Ki67 staining, while low-grade lesions and normal cells were negative. Sec62/Ki67 staining was highly sensitive and specific for the detection of CIN2+ and CIN3+ lesions (94.37%; 100% and 100%; 84.09%, respectively).
Conclusions: Sec62/Ki67 dual-staining immunocytochemistry is a promising cytological tool for interpreting high-grade squamous lesions in cytological specimens and for assessing the risk of progression to cancer.
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| http://dx.doi.org/10.1007/s00404-018-4981-4 | DOI Listing |
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Article Synopsis
- The study investigates the role of Sec62 in cancer, showing its importance in tumor cell migration and stress tolerance.
- Researchers performed Sec62/Ki67 dual staining on 100 cervical cytology specimens to identify high-grade dysplasia and cancer.
- The results indicated that all high-grade cervical lesions (CIN3+) tested positive for the staining, demonstrating its effectiveness and reliability in detecting high-grade dysplastic lesions and assessing cancer risk.
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