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Multiplexed immunoassay using post-synthesis functionalized hydrogel microparticles. | LitMetric

Multiplexed immunoassay using post-synthesis functionalized hydrogel microparticles.

Lab Chip

Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.

Published: December 2018

AI Article Synopsis

  • Multiplex immunoassay platforms are gaining popularity for detecting multiple proteins in one sample, and the use of hydrogel microparticles made via flow lithography is a key development in this field.* -
  • Traditional methods of attaching antibodies to these microparticles during synthesis can lead to issues like aggregation, making them difficult to use in practice.* -
  • This study introduces a new approach, where antibodies are attached to the hydrogels after they are made, improving their stability and loading capacity, leading to better detection performance compared to conventional ELISA tests.*

Article Abstract

In response to a growing demand for simultaneous detection of multiple proteins in a single sample, multiplex immunoassay platforms have emerged at the forefront of proteomic analysis. In particular, detections using graphically encoded hydrogel microparticles synthesized via flow lithography have received attention for integrating a hydrogel, a substrate that can provide enhanced kinetics and high loading capacity, into the bead-based multiplex platform. Currently, the method of microparticle functionalization involves copolymerization of antibodies with the gel during particle synthesis. However, its practical operation is too precarious to be adopted because antibodies are susceptible to aggregation due to incompatibility with hydrophobic photoinitiators used in the photo-induced gel polymerization. In this work, we present a multiplex immunoassay platform that uses encoded hydrogel microparticles that are functionalized after particle synthesis by conjugating antibodies with remnant active groups readily available in the hydrogels. The method not only precludes antibody aggregation but also augments the loading density of the antibodies, which translates into enhanced detection performance. In addition to multiplexing, our platform demonstrates high sensitivity, a broad assay range, and a fast detection rate that outperform the enzyme linked immunosorbent assay (ELISA).

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Source
http://dx.doi.org/10.1039/c8lc01160eDOI Listing

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