Retraction of 'Polymer nanodiscs and macro-nanodiscs of a varying lipid composition' by Venkata Sudheer Kumar Ramadugu et al., Chem. Commun., 2017, 53, 10824-10826.
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http://dx.doi.org/10.1039/c8cc90544d | DOI Listing |
Langmuir
January 2022
Biophysics and Department of Chemistry, Biomedical Engineering, Macromolecular Science and Engineering, The University of Michigan, Ann Arbor, Michigan 48109-1055, United States.
Recent developments in lipid nanodisc technology have successfully overcome the major challenges in the structural and functional studies of membrane proteins and drug delivery. Among the different types of nanodiscs, the use of synthetic amphiphilic polymers created new directions including the applications of solution and solid-state NMR spectroscopy. The ability to magnetically align large-size (>20 nm diameter) polymer nanodiscs and flip them using paramagnetic lanthanide ions has enabled high-resolution studies on membrane proteins using solid-state NMR techniques.
View Article and Find Full Text PDFLangmuir
February 2020
Biophysics Program and Department of Chemistry, Macromolecular Science and Engineering, Biomedical Engineering , University of Michigan, Ann Arbor , Michigan 48109-1055 , United States.
The ability of amphipathic polymers to self-assemble with lipids and form nanodiscs has been a boon for the field of functional reconstitution of membrane proteins. In a field dominated by detergent micelles, a unique feature of polymer nanodiscs is their much-desired ability to align in the presence of an external magnetic field. Magnetic alignment facilitates the application of solid-state nuclear magnetic resonance (NMR) spectroscopy and aids in the measurement of residual dipolar couplings via well-established solution NMR spectroscopy.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
October 2019
Department of Chemistry and Biophysics, Biomedical Engineering, Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI, 48109-1055, USA.
Experimentally measured residual dipolar couplings (RDCs) are highly valuable for atomic-resolution structural and dynamic studies of molecular systems ranging from small molecules to large proteins by solution NMR spectroscopy. Here we demonstrate the first use of magnetic-alignment behavior of lyotropic liquid-crystalline polymer macro-nanodiscs (>20 nm in diameter) as a novel alignment medium for the measurement of RDCs using high-resolution NMR. The easy preparation of macro-nanodiscs, their high stability against pH changes and the presence of divalent metal ions, and their high homogeneity make them an efficient tool to investigate a wide range of molecular systems including natural products, proteins, and RNA.
View Article and Find Full Text PDFChem Phys Lipids
March 2019
Biophysics Program, The University of Michigan, Ann Arbor, MI 48109-1055, USA; Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA; Biomedical Engineering, The University of Michigan, Ann Arbor, MI 48109-1055, USA; Macromolecular Science and Engineering, The University of Michigan, Ann Arbor, MI 48109-1055, USA. Electronic address:
There is considerable interest in the development of membrane mimetics to study the structure, dynamics and function of membrane proteins. Polymer nanodiscs have been useful as a membrane mimetic by not only providing a native-like membrane environment, but also have the ability to extract the desired membrane protein directly from the cell membrane. In spite of such great potential, polymer nanodiscs have their disadvantages including lack of size control and instability at low pH and with divalent metals.
View Article and Find Full Text PDFChem Commun (Camb)
December 2018
Biophysics Program and Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA.
Retraction of 'Polymer nanodiscs and macro-nanodiscs of a varying lipid composition' by Venkata Sudheer Kumar Ramadugu et al., Chem. Commun.
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