AI Article Synopsis
- Synchrotron hydroxyl radical footprinting is a novel structural technique to study nucleic acids and proteins, developed in the late '90s and advanced at Lawrence Berkeley National Laboratory.
- The method focuses on assessing structural features and conformational changes in biological samples in solution, with ongoing enhancements in instrumentation.
- Future improvements, like real-time irradiation and inline fluorescence monitoring, will allow for faster dose response evaluations and broaden the scope of biological research possible with this technique.
Article Abstract
Background: Synchrotron hydroxyl radical footprinting is a relatively new structural method used to investigate structural features and conformational changes of nucleic acids and proteins in the solution state. It was originally developed at the National Synchrotron Light Source at Brookhaven National Laboratory in the late nineties, and more recently, has been established at the Advanced Light Source at Lawrence Berkeley National Laboratory. The instrumentation for this method is an active area of development, and includes methods to increase dose to the samples while implementing high-throughput sample delivery methods.
Conclusion: Improving instrumentation to irradiate biological samples in real time using a sample droplet generator and inline fluorescence monitoring to rapidly determine dose response curves for samples will significantly increase the range of biological problems that can be investigated using synchrotron hydroxyl radical footprinting.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655235 | PMC |
| http://dx.doi.org/10.2174/0929866526666181128125725 | DOI Listing |
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