Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test.

Open Vet J

Laboratory of production and development of viral veterinary vaccines, Institut Pasteur of Algeria. Annex of Kouba, 01 rue de caire, kouba, Algiers, Algeria.

Published: September 2018

Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post-vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody titre (T) respectively. The authors have demonstrated that a positive correlation exists between N.I. and T values, this study aimed to add more evidence to this correlation by establishing a graph and its mathematical equations. We found that: N.I. = (1.489 Log T) + 1.331; this serves as a base to calculate N.I. using T values measured by beta-VNT without going through alpha-VNT and vice versa. At the end of this study, we evaluated the equation accuracy by two parameters; the deviation () and the error percentage, which were = 0.2 and error (%) = 8%, respectively.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172408PMC
http://dx.doi.org/10.4314/ovj.v8i3.15DOI Listing

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