New Nodule Type Found in the Lungs of , an Intermediate Host of .

Background: () lung nodules, were commonly caused by infection. Here, we found a new nodule type without any parasites.

Methods: Overall, 447 P. canaliculata snails were collected in Ning Bo, Zhe Jiang, China in 2018. In order to exhibit the similarities and differences between two nodules types (2018, Huzhou Zhejiang, China), both types were collected in formalin for tissue pathological sectioning. Besides, to obtain the microbial community of the new nodule, the 18S ribosomal RNA (rRNA) gene of it was amplified and analyzed using the Illumina second-generation sequencing platform.

Results: Although two nodules were found in the lungs of , they were different in shape and pathology. Illumina sequencing indicated sp., a species of golden algae, might be the causing agent of the new nodule.

Conclusion: We firstly found a new pathological nodule type in the lungs of , and this nodule might be induced by golden algae infection, however, the direct link between the golden algae and the new nodules, as well as the nodules' impact on the snails' physiology and infection require further study.