The effect of the low temperature (4 ºC) on the orga-nization of actin filaments (microfilaments) of cells of different growth zones of the root of Arabidopsis thaliana (L.) have been studied. It was found that cold treatment inhibits growth of the main root and gives its morphology, causing a large number of deformed (ectopic) root hairs in the zone of differentiation. The temporal relationship of the disorientation and the organization of actin filaments and the detected changes of growth and morphology of roots under conditions of cold factor is shown. It has been found that the most sensitive to the cold are actin filaments of root hairs, meristematic cells, cells of elongation zone, and all epidermal cells of the root zones of A. thaliana.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Structural changes in troponin during activation of skeletal and heart muscle determined in situ by polarised fluorescence.
Biophys Rev
December 2024
Randall Centre for Cell & Molecular Biophysics, New Hunt's House, Guy's Campus, King's College London, London, UK.
Calcium binding to troponin triggers the contraction of skeletal and heart muscle through structural changes in the thin filaments that allow myosin motors from the thick filaments to bind to actin and drive filament sliding. Here, we review studies in which those changes were determined in demembranated fibres of skeletal and heart muscle using fluorescence for in situ structure (FISS), which determines domain orientations using polarised fluorescence from bifunctional rhodamine attached to cysteine pairs in the target domain. We describe the changes in the orientations of the N-terminal lobe of troponin C (TnC) and the troponin IT arm in skeletal and cardiac muscle cells associated with contraction and compare the orientations with those determined in isolated cardiac thin filaments by cryo-electron microscopy.
View Article and Find Full Text PDFUnlabelled: Myosin-IC (myo1c) is a class-I myosin that supports transport and remodeling of the plasma membrane and membrane-bound vesicles. Like other members of the myosin family, its biochemical kinetics are altered in response to changes in mechanical loads that resist the power stroke. However, myo1c is unique in that the primary force-sensitive kinetic transition is the isomerization that follows ATP binding, not ADP release as in other slow myosins.
View Article and Find Full Text PDFPolarity sorting of actin filaments by motor-driven cargo transport.
Biophys J
January 2025
Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996, USA. Electronic address:
During the active transport of cellular cargo, forces generated by cargo-associated molecular motors propel the cargo along cytoskeletal tracks. However, the forces impact not only the cargo, but also the underlying cytoskeletal filaments. To better understand the interplay between cargo transport and the organization of cytoskeletal filaments, we employ coarse-grained computer simulations to study actin filaments interacting with cargo-anchored myosin motors in a confined domain.
View Article and Find Full Text PDFTwo ligands of Arp2/3 complex, yeast coronin and GMF, interact and synergize in pruning branched actin networks.
J Biol Chem
January 2025
Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, MA, USA. Electronic address:
The rapid turnover of branched actin networks underlies key in vivo processes such as lamellipodial extension, endocytosis, phagocytosis, and intracellular transport. However, our understanding of the mechanisms used to dissociate, or 'prune', branched filaments has remained limited. Glia maturation factor (GMF) is a cofilin family protein that binds to Arp2/3 complex and catalyzes branch dissociation.
View Article and Find Full Text PDFBiofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction.
J Nanobiotechnology
January 2025
School of Medicine, Shanghai University, Shanghai, 200444, China.
Biochips are widely applied to manipulate the geometrical morphology of stem cells in recent years. Patterned antenna-like pseudopodia are also probed to explore the influence of pseudopodia formation on gene delivery and expression on biochips. However, how the antenna-like pseudopodia affect gene transfection is unsettled and the underlying trafficking mechanism of exogenous genes in engineered single cells is not announced.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!