Nuclear/Cytoplasmic Fractionation of Proteins from .

Bio Protoc

European Research Institute for the Biology of Ageing, University of Groningen, University Medical Centre Groningen, Groningen, the Netherlands.

Published: October 2018

is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in lysates, which can give insight into the location, function and/or toxicity of proteins.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245569PMC
http://dx.doi.org/10.21769/BioProtoc.3053DOI Listing

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