Lens-free microscopy multispectral acquisitions are processed with an inverse problem approach: a multispectral total variation criterion is defined and minimized with the conjugate gradients method. Reconstruction results show that the method is efficient to recover the phase image of densely packed cells.
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| http://dx.doi.org/10.1364/BOE.9.005828 | DOI Listing |
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View Article and Find Full Text PDFArticle Synopsis
- The SSLFPR method enables high-resolution phase imaging using a lens-free approach and LED illumination by efficiently retrieving phase information from a single diffraction pattern.* -
- By utilizing quasi-monochromatic light components and a virtual wavelength scanning approach, the system addresses resolution loss and improves imaging quality, achieving a half-width resolution of 977 nm over a large field-of-view.* -
- The method's ability to capture real-time images of dynamic samples, like living HeLa cells, positions SSLFPR as a promising technology for various biological and medical applications due to its simplicity and high throughput.*
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