Mimicking Nature by biocatalytic cascade reactions in a whole-cell environment is a revolutionary development in multistep synthesis for the production of bulk and fine chemicals. In the past decade, several proof of concept success stories demonstrated the power of those synthetic cascades and paved the road for future industrial applications. Although enzymes and their promiscuity are best suited to construct such artificial pathways, the complexity and the lack of understanding of the cellular machinery slowed down this progress significantly. In this review, recent achievements in the field of whole-cell biocatalysis are described, challenges and hidden traps that have to be overcome are depicted, and strategies are illustrated how to increase overall cascade productivity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cbpa.2018.10.016 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Nitrate/Nitrite Biosensor Designed with an Antiterminator for Diagnosis of Colitis Based on .
ACS Synth Biol
January 2025
Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, P.R. China.
is a common microorganism in the human gut that has been linked to health benefits. Furthermore, it is an emerging synthetic biology chassis with the potential to be modified into diagnostic or therapeutic engineered probiotics. However, the absence of biological components limits its further applications.
View Article and Find Full Text PDFWhole-cell aptamer-based techniques for rapid bacterial detection: Alternatives to traditional methods.
J Pharm Biomed Anal
January 2025
University Grenoble Alpes, DPM UMR 5063, CNRS, Grenoble F-38041, France. Electronic address:
Article Synopsis
- Bacterial infectious diseases pose a significant public health challenge, especially with rising antibiotic resistance, making fast detection of pathogens essential for control measures.
- Current methods rely on culture techniques, which are time-consuming and require special equipment, leading to a need for alternative detection methods that can quickly identify whole bacteria without culture.
- Aptamer-based assays are emerging as a promising alternative, offering benefits such as stability, specificity, and cost-effectiveness, with various detection techniques like optical and electrochemical methods enhancing their utility in diagnosing bacterial infections.
Evaluation of the cross-immunity between Mycobacterium tuberculosis and Mycobacterium abscessus in vitro.
BMC Microbiol
January 2025
School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, China.
Mycobacterium tuberculosis (M. tuberculosis) and Mycobacterium abscessus (M. abscessus) are important pathogens that can cause lung diseases.
View Article and Find Full Text PDFDecoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy.
Science
January 2025
Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Würzburg, Germany.
Elucidating the interaction between membrane proteins and antibodies requires whole-cell imaging at high spatiotemporal resolution. Lattice light-sheet (LLS) microscopy offers fast volumetric imaging but suffers from limited spatial resolution. DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT) achieves molecular resolution but is restricted to two-dimensional imaging owing to long acquisition times.
View Article and Find Full Text PDFArtificial metalloenzyme assembly in cellular compartments for enhanced catalysis.
Nat Chem Biol
January 2025
State Key Laboratory of Chemo/Biosensing and Chemometrics and School of Chemistry and Chemical Engineering, Hunan University, Changsha, China.
Artificial metalloenzymes (ArMs) integrated within whole cells have emerged as promising catalysts; however, their sensitivity to metal centers remains a systematic challenge, resulting in diminished activity and turnover. Here we address this issue by inducing in cellulo liquid-liquid phase separation through a self-labeling fusion protein, HaloTag-SNAPTag. This strategy creates membraneless, isolated liquid condensates within Escherichia coli as protective compartments for the assembly of ArMs using the same fusion protein.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!