AI Article Synopsis
- Ceramide is important for processes like autophagy (cell recycling), inflammation, and apoptosis (cell death), but its molecular functions aren't fully understood.
- Researchers used azide-tagged sphingolipids to effectively identify targets that interact with ceramide.
- Through protein array analysis and mass spectrometry, they discovered both known ceramide-binding proteins and new ones, validating several using different experimental techniques.
Article Abstract
Ceramide plays key roles in autophagy, inflammation and apoptosis. However, little is known about the molecular mechanisms regulating its function and only a handful of cellular effectors are known for this lipid. Here we show that azide-tagged sphingolipids are powerful tools to identify ceramide targets. The combination of a protein array analysis and a mass spectrometry-based proteomic profiling successfully detects known ceramide-binding proteins and identifies others not yet reported, several of which we validated using a variety of techniques.
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| http://dx.doi.org/10.1039/c8cc05691a | DOI Listing |
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