AI Article Synopsis

  • A9 cells were tested for the effects of 10 µM uranyl nitrate, comparing exposed bacteria to control groups over time intervals of 0.5, 4, and 24 hours.
  • High-throughput proteomics analysis was conducted using advanced mass spectrometry to identify protein changes due to uranium exposure, utilizing a protein sequence database created from genome sequencing data.
  • The findings contribute to a broader study on how a specific Chernobyl bacterial isolate responds to uranium stress, with data archived for further reference.

Article Abstract

A9 cells were exposed or not to 10 µM uranyl nitrate as resting cells in sodium chloride solution. Bacteria exposed to U(VI) and controls were harvested after 0.5, 4, and 24 h of toxicant exposure. Bacteria were subjected to high-throughput proteomics analysis using a Q-Exactive HF high resolution tandem mass spectrometer incorporating an ultra-high-field orbitrap analyzer. MS/MS spectra were assigned with a protein sequence database derived from a draft genome obtained by Illumina sequencing and systematic six-reading frame translation of all the contigs. Proteins identified in bacteria exposed to U(VI) and controls at the three time points allow defining the proteome dynamics upon uranium stress. The data reported here are related to a published study regarding the proteome dynamics of A9 upon uranium stress by Gallois et al. (in press) entitled "Proteogenomic insights into uranium tolerance of a Chernobyl׳s Microbacterium bacterial isolate". The data accompanying the manuscript describing the database searches and comparative analysis have been deposited to the ProteomeXchange with identifier PXD005794.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231083PMC
http://dx.doi.org/10.1016/j.dib.2018.10.136DOI Listing

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