CrAssphages represent the most abundant virus in the human gut microbiota, but the lack of available genome sequences for comparison has kept them enigmatic. Recently, sequence-based classification of distantly related crAss-like phages from multiple environments was reported, leading to a proposed familial-level taxonomic group. Here, we assembled the metagenomic sequencing reads from 702 human fecal virome/phageome samples and analyzed 99 complete circular crAss-like phage genomes and 150 contigs ≥70 kb. In silico comparative genomics and taxonomic analysis enabled a classification scheme of crAss-like phages from human fecal microbiomes into four candidate subfamilies composed of ten candidate genera. Laboratory analysis was performed on fecal samples from an individual harboring seven distinct crAss-like phages. We achieved crAss-like phage propagation in ex vivo human fecal fermentations and visualized short-tailed podoviruses by electron microscopy. Mass spectrometry of a crAss-like phage capsid protein could be linked to metagenomic sequencing data, confirming crAss-like phage structural annotations.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.chom.2018.10.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Adaptations in gut Bacteroidales facilitate stable co-existence with their lytic bacteriophages.
bioRxiv
November 2024
APC Microbiome Ireland & School of Microbiology, University College Cork, Cork, T12 YT20, Ireland.
Background: Bacteriophages (phages) and bacteria within the gut microbiome persist in long-term stable coexistence. These interactions are driven by eco-evolutionary dynamics, where bacteria employ a variety of mechanisms to evade phage infection, while phages rely on counterstrategies to overcome these defences. Among the most abundant phages in the gut are the crAss-like phages that infect members of the Bacteroidales, in particular In this study, we explored some of the mechanisms enabling the co-existence of four phage-Bacteroidales host pairs using a multi-omics approach (transcriptomics, proteomics and metabolomics).
View Article and Find Full Text PDFPersistence of crAssBcn phages in conditions of natural inactivation and disinfection process and their potential role as human source tracking markers.
Sci Total Environ
December 2024
Department of Genetics, Microbiology and Statistics, Section of Microbiology, Virology and Biotechnology, School of Biology, University of Barcelona, Diagonal 643, E-08028 Barcelona, Spain. Electronic address:
Article Synopsis
- Some crAss-like phages, particularly the crAssBcn group, show promise as indicators of human fecal pollution for tracking microbial sources due to their prevalence in the gut and broad distribution.
- Recent studies evaluated the infectivity and stability of three crAssBcn phages alongside the original crAssphage under various conditions, revealing that they remained infectious for a month at moderate temperatures and pH levels but were highly susceptible to low pH and chlorine treatment.
- The research highlighted that inactivation rates for crAssBcn phages were generally lower than those for their bacterial host, Bacteroides intestinalis, and noted seasonal variations with higher inactivation rates in warm months due to environmental factors like sunlight.
Recruitment of complete crAss-like phage genomes reveals their presence in chicken viromes, few human-specific phages, and lack of universal detection.
ISME J
January 2024
Departament de Genètica, Microbiologia i Estadística, Facultat de Biologia, Universitat de Barcelona, Avinguda Diagonal, 643, Prevosti Building, Floor 0. Barcelona E-08028, Spain.
The order Crassvirales, which includes the prototypical crAssphage (p-crAssphage), is predominantly associated with humans, rendering it the most abundant and widely distributed group of DNA phages in the human gut. The reported human specificity and wide global distribution of p-crAssphage makes it a promising human fecal marker. However, the specificity for the human gut as well as the geographical distribution around the globe of other members of the order Crassvirales remains unknown.
View Article and Find Full Text PDFConvergence of gut phage communities but not bacterial communities following wild mouse bacteriophage transplantation into captive house mice.
ISME J
January 2024
Department of Zoology, Faculty of Science, Charles University, Viničná 7, 128 44, Prague, Czech Republic.
Bacteriophages are abundant components of vertebrate gut microbial communities, impacting bacteriome dynamics, evolution, and directly interacting with the superhost. However, knowledge about gut phageomes and their interaction with bacteriomes in vertebrates under natural conditions is limited to humans and non-human primates. Widely used specific-pathogen-free (SPF) mouse models of host-microbiota interactions have altered gut bacteriomes compared to wild mice, and data on phageomes from wild or other non-SPF mice are lacking.
View Article and Find Full Text PDFDevelopment of a novel crAss-like phage detection method with a broad spectrum for microbial source tracking.
Water Res
November 2024
Department of Food Science & Technology, Chungnam National University, Daejeon, Republic of Korea. Electronic address:
Article Synopsis
- * A study identified 13 CLPs in South Korean human fecal samples, categorizing them into seven genera, with particular focus on genus VI, which showed the highest prevalence compared to other genera.
- * The research emphasizes the potential of CLPs, especially genus VI, as effective microbial source tracking (MST) markers, which could improve hygiene regulations and reduce public health costs in monitoring human waste and environmental samples.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!