A product translated from the 1b gene of raspberry bushy dwarf virus (RBDV) was specifically detected in RBDV-infected Nicotiana benthamiana plants by immunoblot analysis. To analyze the effects of the 1b gene on virus infection in host plants, an RBDV deletion mutant virus (RB∆1bstop), which is unable to express the 1b gene, was constructed and inoculated to N. benthamiana plants. The results showed that accumulation of the virus genomic (g) RNAs 1 and 2 decreased in inoculated leaves, and that systemic virus spread was delayed compared with wild-type RBDV. In contrast, accumulation of the viral gRNAs 1 and 2 was elevated in RB∆1bstop-infected leaf tissues during ectopic expression of the 1b gene. Furthermore, we found that the 1b has weak RNA silencing suppressor activity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.virol.2018.10.025 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assembly and comparative analysis of the complete mitogenome of var. , an exceptional berry plant possessing sweet leaves.
Front Plant Sci
December 2024
Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, College of Life Sciences, Taizhou University, Taizhou, China.
var. is a special berry plant of in the Rosaceae family. Its leaves contain high-sweetness, low-calorie, and non-toxic sweet ingredients, known as rubusoside.
View Article and Find Full Text PDFAmeliorative Effect of Raspberry Ketone on Hypothalamic Inflammation in High Fat Diet-Induced Obese Mice.
J Nutr Sci Vitaminol (Tokyo)
January 2025
Key Laboratory of Sichuan Cuisine Artificial Intelligence, Sichuan Tourism University.
This study aimed to investigate the regulatory effects of raspberry ketone on hypothalamic inflammation and its mechanism. Mouse microglia cells (BV2 cells) were cultured in vitro with palmitic acid (100 μM) to induce inflammation model and then incubated with raspberry ketone (5, 20, 50 μM) alone or raspberry ketone (50 μM) and the specific inhibitor of uncoupling protein 2 (UCP2), genipin (10 μM), to test the role of UCP2 in raspberry ketone regulatory of inflammation. Meanwhile, C57BL/6J mice were fed a high-fat diet containing raspberry ketone (0.
View Article and Find Full Text PDFMicrobiome of honey bee corbicular pollen: Factors influencing its structure and potential for studying pathogen transmission.
Sci Total Environ
January 2025
Institute of Applied Microbiology, Justus-Liebig University Giessen, IFZ, Heinrich-Buff Ring 26, 35392 Giessen, Germany.
Honey bees are exposed to a diverse variety of microbes in the environment. Many studies have been carried out on the microbiome of bee gut, beebread, and flower pollen. However, little is known regarding the microbiome of fresh corbicular pollen, which can directly reflect microbes acquired from the environment.
View Article and Find Full Text PDFThe complete chloroplast genome and phylogenetic analysis of Miq 1861 (Roseaceae).
Mitochondrial DNA B Resour
December 2024
College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, China.
The wild raspberry species Miq 1861 is a promising resource for breeding thermotolerant cultivars. Its complete chloroplast genome spans 155,935 base pairs (bp), featuring the classic quadripartite structure: an 18,729 bp small single-copy region, an 85,662 bp large single-copy region, and two 25,772 bp inverted repeats. A total of 130 genes were identified, including 86 protein-coding, 36 tRNA genes, and 8 rRNA genes.
View Article and Find Full Text PDFThe High-Quality Genome Sequencing and Analysis of Red Raspberry ( L.).
Int J Genomics
November 2024
Department of General Surgery & Bio-Bank of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Red raspberry ( L.), which is an important nutritional source for human health, belongs to fruit crops of the Rosaceae family. Here, we used Pacific Biosciences single-molecule real-time (SMRT) sequencing and high-throughput chromosome conformation capture (Hi-C) sequencing technologies to assemble genomes and reported a high-quality L.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!