Porcine-derived xenogeneic sources for transplantation are a promising alternative strategy for providing organs for treatment of end-stage organ failure in human patients because of the shortage of human donor organs. The recently developed blastocyst or pluripotent stem cell (PSC) complementation strategy opens a new route for regenerating allogenic organs in miniature pigs. Since the eye is a complicated organ with highly specialized constituent tissues derived from different primordial cell lineages, the development of an intact eye from allogenic cells is a challenging task. Here, combining somatic cell nuclear transfer technology (SCNT) and an anophthalmic pig model (), allogenic retinal pigmented epithelium cells (RPEs) were retrieved from an E60 chimeric fetus using blastocyst complementation. Furthermore, all structures were successfully regenerated in the intact eye from the injected donor blastomeres. These results clearly demonstrate that not only differentiated functional somatic cells but also a disabled organ with highly specialized constituent tissues can be generated from exogenous blastomeres when delivered to pig embryos with an empty organ niche. This system may also provide novel insights into ocular organogenesis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6284517 | PMC |
| http://dx.doi.org/10.15252/emmm.201808861 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Three-dimensional design of a geometric model for an ocular prosthesis in ex vivo anophthalmic socket models.
Acta Ophthalmol
March 2021
Department of Ophthalmology, University Hospitals Leuven, Leuven, Belgium.
Purpose: Fitting a customized ocular prosthesis for anophthalmic patients entails an artisanal labour-exhausting process and is standardly based on impression moulding of the socket, which may be anatomically inaccurate. The objective of the study was to design an impression-free socket mould with three-dimensional (3D) technology.
Methods: The ex vivo anophthalmic socket models included one silicone, one fresh pig cadaver head and three fresh-frozen human cadaver heads.
Efficacy of porcine acellular dermal matrix in the management of lower eyelid retraction: case series and review of the literature.
Graefes Arch Clin Exp Ophthalmol
September 2020
Orbital Plastic and Lacrimal Clinic, Royal Victorian Eye and Ear Hospital, 32 Gisborne Street, East Melbourne, VIC, 3002, Australia.
Purpose: To investigate the efficacy of Permacol™, a decellularized porcine dermal-derived membrane, as a spacer in the management of lower eyelid retraction. The efficacy of sizing and insertion was investigated, as well as complications. The literature was also reviewed to compare this material with other porcine-derived grafts in use for the management of lower eyelid retraction.
View Article and Find Full Text PDFGrowth and Behavior of Congenitally Anophthalmic Lee-Sung Pigs.
Comp Med
May 2019
Institute of Veterinary Clinical Science, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
Article Synopsis
- Circadian rhythms are typically influenced by light-dark cycles, and congenitally anophthalmic miniature pigs serve as a unique model to study these rhythms without visual light exposure.
- The study examined growth and behavior in Lee-Sung pigs with congenital anophthalmia (LSP-A) compared to normally developed pigs (LSP-N), finding no differences in growth but notable differences in activity patterns.
- LSP-A pigs were more active during the day and night, exhibiting circadian rhythm abnormalities similar to blind humans, suggesting potential applications for understanding brain plasticity and hormone regulation without visual cues.
Rescuing ocular development in an anophthalmic pig by blastocyst complementation.
EMBO Mol Med
December 2018
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Porcine-derived xenogeneic sources for transplantation are a promising alternative strategy for providing organs for treatment of end-stage organ failure in human patients because of the shortage of human donor organs. The recently developed blastocyst or pluripotent stem cell (PSC) complementation strategy opens a new route for regenerating allogenic organs in miniature pigs. Since the eye is a complicated organ with highly specialized constituent tissues derived from different primordial cell lineages, the development of an intact eye from allogenic cells is a challenging task.
View Article and Find Full Text PDFSurgical Outcomes of Porcine Acellular Dermis Graft in Anophthalmic Socket: Comparison with Oral Mucosa Graft.
Korean J Ophthalmol
February 2017
Institute of Vision Research, Department of Ophthalmology, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.
Purpose: We describe our experience with the Permacol graft in anophthalmic socket reconstruction, and compare it to the autologous buccal mucosal graft, emphasizing the postoperative vascularization and contraction of each graft.
Methods: This was a retrospective comparative study. We measured the time necessary for the graft surface to be completely vascularized, as well as the fornix depth of the conjunctival sac in anophthalmic patients.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!