Quantification of withaferin-A and withanolide-A in diploid ( = 12) and tetraploid cytotypes ( = 24) of "Rassbhary", L.

During the present study an analytical method based on reverse-phase high performance liquid chromatography photodiode array detection method was developed for simultaneous determination of withaferin-A and withanolide-A in plant parts of two cytotypes (diploid  = 12 & tetraploid  = 24) of . All the plant parts were extracted in different solvent solutions i.e., acidic [HCl] methanol (i.e., methanol containing 0.3% of HCl), methanol, -hexane, chloroform. Both the compounds were comparatively analysed. The results revealed that tetraploid cytotype ( = 24) showed the higher composition of both the reference compounds. The method is simple, rapid and provides better resolution can be easily applied to the quantitative analyses of withanolides in plant matrices.