Objectives: Although it has been regarded that the CTX-M-type extended-spectrum β-lactamase (ESBL) gene bla is mainly carried by antimicrobial resistance plasmids, Escherichia coli possessing chromosomally-located bla has been reported in previous studies. This study aimed to characterise the genetic structure of the chromosomally-located bla transposition unit and its surrounding sequence in ESBL-producing E. coli isolated in a Japanese hospital.
Methods: A total of 81 ESBL-producing E. coli isolates were studied. The existence of chromosomally-located bla was confirmed by S1 nuclease-digested pulsed-field gel electrophoresis (PFGE) and Southern blot hybridisation and by sequencing analysis of the PCR-amplified DNA fragments.
Results: Chromosomally-located bla was confirmed in 22 (27.2%) of the 81 E. coli isolates examined; five and four location types of chromosomally-located bla and bla were determined, respectively. Among the 22 E. coli isolates, 15 (68.2%) possessed single chromosomally-located bla gene, probably due to single transposition of a plasmidic bla to the chromosome. In isolate N0057, the bla transposition unit was transferred from a plasmid into two different chromosomal regions. In addition, 'recurrent' transposition of already existing chromosomally-located bla to another chromosomal region was observed in isolates N0211, N0214, N01127, N1682 and N1753; consequently, these isolates possessed two copies of chromosomally-located bla.
Conclusion: Considering that isolates N0211, N0214, N01127, N1682 and N1753 in which the 'recurrent' transposition event occurred were genetically related according to PFGE, these data suggest the possibility of accumulation of bla on the chromosome in CTX-M-type ESBL-producing E. coli.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jgar.2018.11.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chromosomally located bla in Enterobacter cloacae complex across human-bird-environment interfaces: A one-health perspective.
Sci Total Environ
December 2024
Department of Animal Health and Food Safety, School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address:
To improve the understanding of antimicrobial resistance (AMR) in migratory birds derived-Enterobacter cloacae (E. cloacae) complex and its spread at the human-bird-environment interface, we isolated 11 strains of third-generation cephalosporin-resistant E. cloacae from 1003 specimens from 29 migratory bird species over two years in Chongming, Shanghai, China.
View Article and Find Full Text PDFVIM-1-producing Enterobacter asburiae with mobile colistin resistance genes from wastewaters.
BMC Genomics
September 2024
Central European Institute of Technology, University of Veterinary Sciences Brno, Brno, Czech Republic.
Background: Wastewaters are considered as important players in the spread of antimicrobial resistance, thus affecting the health of humans and animals. Here, we focused on wastewaters as a possible source of carbapenemase-producing Enterobacterales for the environment.
Methods: A total of 180 presumptive coliforms from hospital and municipal wastewaters, and a river in the Czech Republic were obtained by selective cultivation on meropenem-supplemented media and tested for presence of carbapenemase-encoding genes by PCR.
Characterization of the Chromosomally Located Metallo--Lactamase Genes and in a Carbapenem-Resistant Clinical Isolate.
Microb Drug Resist
October 2024
Department of Laboratory Diagnostics, Changhai Hospital, Naval Medical University, Shanghai, China.
Characterization of the multidrug resistance (MDR) region in strain PA59 revealed the presence of antibiotic resistance genes, including and , within a complex genetic landscape of mobile genetic elements. Carbapenem-resistant (CRPA) strains were isolated from Shanghai Changhai Hospital. Polymerase chain reaction (PCR) was used to detect the -lactamase genes in the isolated strains.
View Article and Find Full Text PDFPrevalence and Genomic Characterization of Multidrug-Resistant Serovar Kentucky Sequence Type 198 Circulating - Beijing Municipality, China, 2016-2023.
China CDC Wkly
August 2024
Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing, China.
Article Synopsis
- - The study focuses on a highly resistant strain of Salmonella Kentucky (ST198) that poses a global health threat due to its multidrug resistance, particularly against fluoroquinolones.
- - Researchers analyzed 54 Kentucky isolates from clinical and food sources in Beijing between 2016 and 2023 using genome sequencing and antibiotic testing, revealing various resistance patterns and mutations.
- - Findings show significant genetic diversity within the Kentucky population, highlighting the need for enhanced monitoring and control strategies to combat antimicrobial resistance (AMR) in both food and human health contexts.
Occurrence of Carbapenemase in ST2519 of Clinical Origin: A Report from Northeast India.
Microb Drug Resist
October 2024
Department of Microbiology, Assam University, Silchar, India.
Carbapenem-resistant pose a significant threat to global public health due to the dearth of available treatment options, resulting in infections with high mortality and morbidity. The study aimed to investigate the mechanism of carbapenem resistance in a carbapenem non-susceptible isolate recovered from an urinary tract infection patient admitted to a tertiary referral hospital, through whole-genome sequencing using Illumina NovaSeq 6000 platform. Carbapenemase production followed by antibiotic susceptibility testing were performed following Clinical Laboratory Standard Institute guidelines.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!