AI Article Synopsis
- A study was conducted on single-particle imaging (SPI) of the PR772 bacteriophage at the AMO beamline of LCLS, focusing on developing a workflow for creating a 3D reconstruction of the virus from collected data.
- The workflow includes steps like classifying single-hit diffraction data, refining that data, determining orientations for 3D scattering intensity, and reconstructing the virus's electron density without symmetry constraints.
- The analysis achieved a resolution of better than 10 nm, revealing nanoscale features and showing that the virus structure is compressed in one direction, deviating from the typical icosahedral symmetry.
Article Abstract
The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211532 | PMC |
| http://dx.doi.org/10.1107/S205225251801120X | DOI Listing |
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