The novel cry52Ca1 gene from an Indian Bacillus thuringiensis (Bt) isolate was cloned in an expression vector (pET301/CT-DEST, 6xHis). The gene expressed as a ∼77.2 kDa protein in E. coli BL21-CodonPlus (DE3)-RIPL cells upon induction with isopropyl-thio-galactoside (IPTG) for 18 h at 28 °C. Cry52Ca1 protein was toxic to Helicoverpa armigera (cotton bollworm) neonate larvae (LC 36.66 µg per ml and MIC 3.051 µg per ml) in diet-based laboratory assays. This gene has potential for deployment in insect-resistant transgenic crops.
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A novel cry52Ca1 gene from an Indian Bacillus thuringiensis isolate is toxic to Helicoverpa armigera (cotton boll worm).
J Invertebr Pathol
November 2018
ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi 110012, India. Electronic address:
The novel cry52Ca1 gene from an Indian Bacillus thuringiensis (Bt) isolate was cloned in an expression vector (pET301/CT-DEST, 6xHis). The gene expressed as a ∼77.2 kDa protein in E.
View Article and Find Full Text PDFPool deconvolution approach for high-throughput gene mining from Bacillus thuringiensis.
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ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, 110012, India.
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