Bacillus salidurans sp. nov., isolated from salt-accumulated pepper rhizospheric soil.

A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea. Cell growth of strain KNUC7312 occurred at 10-45 °C (optimum, 30 °C) and pH 7-12 (optimum, pH 7). In addition, this strain was able to tolerate 0-12 % NaCl (w/v) concentration (optimum, 0-1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312 clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318 (98.0 %). The predominant respiratory quinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0. The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid. Strain KNUC7312 showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318, which supported that this strain represents a novel Bacillusspecies. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312 represents a novel species within the genera Bacillus. The name Bacillus salildurans sp. nov. is proposed. The type strain is KNUC7312 (KCTC 33852=CGMCC 1.13629).