AI Article Synopsis
- Two-component systems in microorganisms involve a receptor histidine kinase and a response regulator that control essential cellular processes.
- The study introduces a new method to identify important amino acids in DesK, a thermosensor from Bacillus subtilis, by using random mutagenesis and observing changes during sporulation.
- This approach not only helps in finding mutants with defective temperature sensing in DesK but can also be extended to study other histidine kinases and develop new reporter assays for various regulatory systems.
Article Abstract
Two component systems, composed of a receptor histidine kinase and a cytoplasmic response regulator, regulate pivotal cellular processes in microorganisms. Here we describe a new screening procedure for the identification of amino acids that are crucial for the functioning of DesK, a prototypic thermosensor histidine kinase from Bacillus subtilis. This experimental strategy involves random mutagenesis of the membrane sensor domain of the DesK coding sequence, followed by the use of a detection procedure based on changes in the colony morphogenesis that take place during the sporulation programme of B. subtilis. This method permitted us the recovery of mutants defective in DesK temperature sensing. This screening approach could be applied to all histidine kinases of B. subtilis and also to kinases of other bacteria that are functionally expressed in this organism. Moreover, this reporter assay could be expanded to develop reporter assays for a variety of transcriptionally regulated systems.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/mic.0.000741 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Protein dynamics underlies strong temperature dependence of heat receptors.
Proc Natl Acad Sci U S A
January 2025
Department of Physiology and Biophysical Sciences, State University of New York at Buffalo, Buffalo, NY 14214.
Ion channels are generally allosteric proteins, involving specialized stimulus sensor domains conformationally linked to the gate to drive channel opening. Temperature receptors are a group of ion channels from the transient receptor potential family. They exhibit an unprecedentedly strong temperature dependence and are responsible for temperature sensing in mammals.
View Article and Find Full Text PDFOn-Chip Metamaterial-Enhanced Mid-Infrared Photodetectors with Built-In Encryption Features.
Adv Sci (Weinh)
January 2025
College of Physics and Optoelectronic Engineering, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, No. 1, Sub-Lane Xiangshan, Xihu District, Hangzhou, 310024, China.
The integration of mid-infrared (MIR) photodetectors with built-in encryption capabilities holds immense promise for advancing secure communications in decentralized networks and compact sensing systems. However, achieving high sensitivity, self-powered operation, and reliable performance at room temperature within a miniaturized form factor remains a formidable challenge, largely due to constraints in MIR light absorption and the intricacies of embedding encryption at the device level. Here, a novel on-chip metamaterial-enhanced, 2D tantalum nickel selenide (Ta₂NiSe₅)-based photodetector, meticulously designed with a custom-engineered plasmonic resonance microstructure to achieve self-powered photodetection in the nanoampere range is unveiled.
View Article and Find Full Text PDFAmplicon Sequencing Reveals Diversity in Spatially Separated Microbial Communities in the Icelandic Mars Analog Environment Mælifellssandur.
Astrobiology
January 2025
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Exploration missions to Mars rely on landers or rovers to perform multiple analyses over geographically small sampling regions, while landing site selection is done using large-scale but low-resolution remote-sensing data. Utilizing Earth analog environments to estimate small-scale spatial and temporal variation in key geochemical signatures and biosignatures will help mission designers ensure future sampling strategies meet mission science goals. Icelandic lava fields can serve as Mars analog sites due to conditions that include low nutrient availability, temperature extremes, desiccation, and isolation from anthropogenic contamination.
View Article and Find Full Text PDFN-Doped Carbon Nanodots as Temperature Sensors and Fluorescent Probes for the Detection of Tinidazole in Milk.
J Fluoresc
January 2025
School of Science, Jiangnan University, Wuxi, 214122, China.
In this study, nitrogen-doped carbon nanodots (N-CDs) with temperature and fluorescence sensing were prepared via hydrothermal method using L-lysine and ethylenediamine as precursors. The synthesized N-CDs exhibited spherical morphology with sizes ranging from 2.8 to 5.
View Article and Find Full Text PDFFiber Optic Micro-Hole Salinity Sensor Based on Femtosecond Laser Processing.
Nanomaterials (Basel)
January 2025
School of Artificial Intelligence, Optics and Electronics (iOPEN), Northwestern Polytechnical University, Xi'an 710072, China.
This study presents a novel reflective fiber Fabry-Perot (F-P) salinity sensor. The sensor employs a femtosecond laser to fabricate an open liquid cavity, facilitating the unobstructed ingress and egress of the liquid, thereby enabling the direct involvement of the liquid in light transmission. Variations in the refractive index of the liquid induce corresponding changes in the effective refractive index of the optical path, which subsequently influences the output spectrum.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!