Objective: The detection accuracy of sentinel lymph node (SLN) metastasis by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) for endometrial cancer (EC) remains unclear and was assessed in this preliminary study.
Methods: We studied primary cancer tissues and pelvic lymph nodes (PLN) from 105 patients with EC. qRT-PCR assay was performed to determine the copy numbers of CK19 mRNA in EC tissues, and negative and positive LN samples. Further, qRT-PCR results were compared with pathological findings.
Results: CK19 mRNA expression was detected in 98% (104/106) of the tumors, with a median copy number of 3.0 × 105/μL. Twelve LN were diagnosed as positive by pathological examination. The median copy number of CK19 mRNA for positive and negative LN was 8.1 × 104/μL and 90.4/µL, respectively. CK19 mRNA expression was higher in pathologically positive LN than in pathologically negative LN (p < 0.01); the pathological and qRT-PCR findings showed no discrepancy. When the cutoff value was set at 4,500 copies/µL, qRT-PCR assay using CK19 mRNA exhibited high sensitivity and specificity.
Conclusions: Our results demonstrated that qRT-PCR assay, using CK19 mRNA, exhibits a high accuracy for detecting LN metastasis in EC and represents a useful alternative to conventional pathological diagnosis of EC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1159/000493485 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reprogramming canine cryopreserved hepatocytes to hepatic progenitor cells using small molecule compounds.
Regen Ther
June 2024
Laboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Azabu University, Sagamihara City, Kanagawa, Japan.
Introduction: Exploring techniques for differentiating and culturing canine hepatocytes serves as a means to establish systems for liver transplantation and drug metabolism testing. However, establishing consistent methods for culturing stable hepatocytes remains a challenge. Recently, several investigations have shown the reprogramming of mature hepatocytes into hepatic progenitor cells by applying specific small molecule compounds, including Y-27632, (a ROCK inhibitor), A-83-01 (a TGFβ inhibitor), and CHIR99021 (a GSK3 inhibitor) (termed YAC) in rat, mouse, and humans, respectively.
View Article and Find Full Text PDFSerum mRNA levels of cytokeratin-19 and vascular endothelial growth factor in oral squamous cell carcinoma and oral potentially malignant disorders using RT-PCR.
BMC Oral Health
September 2024
Faculty of Dentistry, University of Puthisastra, Phnom Penh, Cambodia.
Article Synopsis
- Oral cancers, particularly oral squamous cell carcinoma (OSCC), are increasingly prevalent globally, especially in South-Central Asia, prompting research to find early detection methods through blood biomarkers.
- The study analyzed the levels of Vascular Endothelial Growth Factor-A (VEGF-A) and Cytokeratin-19 (CK-19) mRNAs in blood samples from 40 patients and controls, using real-time RT-PCR to evaluate their potential as early indicators of OSCC.
- Results showed significantly higher levels of CK-19 and VEGF-A in patients with OSCC and related conditions compared to healthy controls, demonstrating a 60% predictive probability for OSCC detection when both markers are used together.
Protective effects of engineered Lactobacillus crispatus strains expressing G-CSF on thin endometrium of mice.
Hum Reprod
October 2024
Reproductive Immunology Laboratory, Basic Medical College, Jilin Medical University, Jilin, China.
Study Question: Does recombinant Lactobacillus expressing granulocyte colony-stimulating factor (G-CSF) have a better protective effect than the current treatment of thin endometrium (TE)?
Summary Answer: This study suggested that the intrauterine injection of Lactobacillus crispastus (L. crispastus)-pPG612-G-CSF has a positive effect on preventing TE induced by 95% alcohol in mice.
What Is Known Already: TE has a negative impact on the success rate of ART in patients, and is usually caused by intrauterine surgery, endometrial infection, or hormone drugs.
Prognostic Significance of Circulating and Disseminated Tumor Cells in Breast Cancer Patients before and after Adjuvant Chemotherapy.
Cell J
July 2024
Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. Email:
Objective: Despite the advances in treatment, breast cancer (BC) remains a major cause of death in women. This study aims to evaluate the prognostic significance of detecting circulating tumor cells (CTCs) and disseminated tumor cells (DTCs) in paired peripheral blood (PB) and bone marrow (BM) samples obtained both before and after adjuvant chemotherapy from patients with operable BC.
Materials And Methods: In this experimental study, from 160 patients with primary BC, we collected 160 PB and BM samples before and we could be able to collect PB and BM samples from 100 of them after adjuvant chemotherapy.
Human amnion mesenchymal stem cells promote endometrial repair via paracrine, preferentially than transdifferentiation.
Cell Commun Signal
May 2024
Department of Obstetrics and Gynecology, The University-Town Hospital of Chongqing Medical University, No. 55, Daxuecheng Middle Road, Chongqing, 401331, China.
Article Synopsis
- Intrauterine adhesion (IUA) is a severe cause of infertility due to damage to the endometrium, and stem cell therapy presents a potential treatment by either paracrine effects or transdifferentiation.
- The study involved co-culturing human amniotic mesenchymal stem cells (hAMSCs) with transformed endometrial stromal cells and assessing various molecular and histological markers after treatment in an IUA rat model.
- Results showed that hAMSCs significantly reduced collagen and fibronectin levels in damaged cells, improved endometrial gland counts, and decreased fibrosis, particularly noted after 6 days of co-culture.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!