AI Article Synopsis
- Organ renewal involves cell division, differentiation, and loss, and a new platform for live imaging of the adult midgut allows researchers to observe these processes in real-time.
- By creating a window on a living animal, imaging sessions can last 12-16 hours, capturing dynamic cell and tissue behavior in detail.
- The analysis reveals novel cell behaviors, such as the re-orientation of mitotic stem cells and unique mechanisms in daughter cell fate determination and enterocyte extrusion.
Article Abstract
Organ renewal is governed by the dynamics of cell division, differentiation and loss. To study these dynamics in real time, we present a platform for extended live imaging of the adult midgut, a premier genetic model for stem-cell-based organs. A window cut into a living animal allows the midgut to be imaged while intact and physiologically functioning. This approach prolongs imaging sessions to 12-16 hr and yields movies that document cell and tissue dynamics at vivid spatiotemporal resolution. By applying a pipeline for movie processing and analysis, we uncover new and intriguing cell behaviors: that mitotic stem cells dynamically re-orient, that daughter cells use slow kinetics of Notch activation to reach a fate-specifying threshold, and that enterocytes extrude via ratcheted constriction of a junctional ring. By enabling real-time study of midgut phenomena that were previously inaccessible, our platform opens a new realm for dynamic understanding of adult organ renewal.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277200 | PMC |
| http://dx.doi.org/10.7554/eLife.36248 | DOI Listing |
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