AI Article Synopsis
- A melamine modified counter-current chromatography column was created to test its effectiveness in separating compounds like stevioside and rebaudioside A, which have similar partition coefficients.
- Results indicated that the modified column achieved better separation due to intermolecular forces between melamine and the compounds, while the unmodified column failed to separate them.
- This research highlights the column modification method as a promising way to enhance separation capabilities in counter-current chromatography, potentially leading to more efficient separation processes for enantiomer compounds.
Article Abstract
In this work, to further verify and develop the novel counter-current chromatography modified column separate mode, a melamine modified counter-current chromatography column was prepared. Meanwhile, the modified counter-current chromatography column was used to separate stevioside and rebaudioside A with the same partition coefficient in chosen solvent system to evaluate its separation efficiency. The results show that because of the presence of intermolecular forces between melamine and model compounds, better separation could be achieved on the modified column while it's almost impossible to be separated on the unmodified column. So the results of this research further show that column modified method is a possible approach to further increase the separation ability of counter-current chromatography. Take advantage of large sample handing capacity of counter-current chromatography, the mothed may have great potential to be an efficient method of separation and preparation enantiomer compounds.
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| http://dx.doi.org/10.1002/jssc.201800914 | DOI Listing |
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