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Exploring the Link Between Infections and Primary Osteoarthritis: A Next-Generation Metagenomic Sequencing Approach.
Int J Mol Sci
December 2024
Department of Medical Microbiology, Medical University of Warsaw, Chalubinski 5 Str., 02-004 Warsaw, Poland.
This prospective pilot study examined the association between microorganisms and knee osteoarthritis by identifying pathogens in the synovial membrane, synovial fluid, and blood samples from two patients with primary bilateral knee osteoarthritis, using metagenomic next-generation sequencing (mNGS). Intraoperatively, during routine knee arthroplasty procedures, we collected the following 12 samples from each patient: two synovial membrane samples, two synovial fluid samples, and two venous blood samples. After DNA isolation and library construction, each sample was subjected to deep whole-genome sequencing using the DNBSEQT17 platform with the read length PE150 as the default.
View Article and Find Full Text PDFHuman fallopian tube organoids provide a favourable environment for sperm motility.
Hum Reprod
January 2025
IRSD, Université de Toulouse, INSERM, INRAE, ENVT, Univ Toulouse III-Paul Sabatier (UPS), Toulouse, France.
Study Question: Does a human fallopian tube (HFT) organoid model offer a favourable apical environment for human sperm survival and motility?
Summary Answer: After differentiation, the apical compartment of a new HFT organoid model provides a favourable environment for sperm motility, which is better than commercial media.
What Is Known Already: HFTs are the site of major events that are crucial for achieving an ongoing pregnancy, such as gamete survival and competence, fertilization steps, and preimplantation embryo development. In order to better understand the tubal physiology and tubal factors involved in these reproductive functions, and to improve still suboptimal in vitro conditions for gamete preparation and embryo culture during IVF, we sought to develop an HFT organoid model from isolated adult stem cells to allow spermatozoa co-culture in the apical compartment.
Total Pelvic Exenteration with Presacral Fascia and Pelvic Musculature Excision for Pelvic Recurrence of Rectal Cancer.
Ann Surg Oncol
January 2025
Division of Colorectal Surgery, Changzheng Hospital, Navy Medical University, Shanghai, China.
Background: Local relapse has not been eradicated even in the era of total mesorectum excision. Although various approaches have been attempted, R0 resection remains the only potentially curative treatment. PATIENT AND METHODS: A 45-year-old woman with a history of laparoscopic abdominoperineal resection was diagnosed with pelvic recurrence 7 months ago.
View Article and Find Full Text PDFLateralization study of the basal ganglia, thalamus and supplying arteries in healthy individuals based on structure and connectivity analysis using 7.0T MRI.
Neuroimage
January 2025
Department of Radiology, First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.. Electronic address:
The human cerebral cortex is known for its hemispheric specialization, which underpins a variety of functions and activities. However, it is not well understood if similar lateralization exists within the deep gray matter nuclei, such as the basal ganglia (BG) and thalamus, and their associated arteries, including the lenticulostriate arteries (LSAs). To explore this, we analyzed images from 7T MRI scans of 40 healthy young individuals.
View Article and Find Full Text PDFUnveiling Oral Health Differences: Gingival Health and Hygiene in Children With and Without Cleft Lip and Palate.
Cleft Palate Craniofac J
January 2025
Hospital of Craniofacial Anomalies Rehabilitation, University of São Paulo, Bauru, Brazil.
Objective: To compare the oral hygiene and gingival health of children with and without cleft lip and palate.
Design: Cross-sectional comparative study.
Setting: Institutional tertiary referral hospital.
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