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Genetic diversity of rhinoviruses detected at a tertiary hospital in Catalonia (Spain) during the 2014-2017 seasons. | LitMetric

AI Article Synopsis

  • The study aimed to analyze the genetic diversity of rhinovirus (RV) among patients in a Barcelona hospital between 2014 and 2017.
  • Researchers used real-time multiplex RT-PCR to detect RV, focusing on samples with a specific viral load for further molecular identification.
  • Findings indicated a diverse range of RV types, with RV-A being the most common, and variations in circulation patterns and age-related differences in RV infection types.

Article Abstract

Aim: To describe the genetic diversity of rhinovirus (RV) from patients attended at a tertiary hospital in Barcelona (Spain) from October 2014 to May 2017.

Methods: RV detection was performed by real-time multiplex RT-PCR. A specific real-time quantitive retrotranscription PCR (qRT-PCR) was carried out to select those samples (Ct < 35) for molecular characterization based on partial VP4/2 protein.

Results: Phylogenetic characterization revealed proportions of 63% RV-A, 6% RV-B and 31% RV-C (119 different types). RV-A circulated throughout all the study period, with a minor circulation during winter, just when RV-C prevailed. Differences between age medians by RV-specie were reported.

Conclusion: The large genetic diversity of RV detected in our area is described here. The variable cocirculation of multiple RV types is also reported, showing differences by age.

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Source
http://dx.doi.org/10.2217/fmb-2018-0151DOI Listing

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