AI Article Synopsis
- - The study investigated the differentiation of Caco-2 colorectal cancer cells using gene expression analysis and bioimpedance spectroscopy, finding maximum transepithelial electrical resistance (TEER) on day 7, which then decreased but stabilized afterward.
- - Baseline resistance peaked on day 4 and dropped to a low on day 7 before gradually rising over two weeks, suggesting the development of basement membrane components and an apical mucous layer.
- - The research highlighted changes in mucin gene expression (MUC3A/MUC3B and MUC17) alongside reductions in certain microRNAs (miR-21 and miR-622), and it proposes this method for further studies on extracellular matrix formation.
Article Abstract
Differentiation of colorectal cancer Caco-2 cells was assessed using Affymetrix Human Gene 1.0 ST arrays and by the main electrical parameters measured by bioimpedance spectroscopy. Transepithelial electrical resistance (TEER) was maximum on day 7, then decreased by day 11, and remained stable. The baseline resistance was maximum on day 4, minimum on day 7, but then gradually increased over 2 weeks, which can be explained by the formation of the basement membrane components or the apical mucous layer. Caco-2 cells express components of laminin-111 and laminin-511. A synchronous increase in the expression of mucin 3 mRNA (MUC3A/MUC3B) and mucin 17 mRNA (MUC17) and reduced expression of miR-21 and miR-622 microRNA genes were observed. Possible use of the described approach for studying the formation of extracellular matrix is discussed.
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| http://dx.doi.org/10.1007/s10517-018-4283-7 | DOI Listing |
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