AI Article Synopsis
- Date palm is cultivated in dry regions and requires gender identification at the seedling stage due to its dioecious nature.
- A specific SCAR marker linked to male plants was developed using a unique DNA primer that only produced a band in male plants.
- This SCAR marker proved effective in accurately identifying the gender of date palm seedlings, ensuring that only male plants are recognized.
Article Abstract
Date palm ( L.) is cultivated in arid and semiarid regions worldwide. Given the dioecious nature of this plant, gender identification is very important at the seedling stage. Molecular markers are very effective tools that help in gender identification at this stage. A sequence characterized amplified region (SCAR) marker linked to sex-specific regions in the genome of date palm was developed. Of the 300 tested randomly amplified polymorphic DNA (RAPD) primers, only one primer (OPC-06) produced reproducible band (294 bp) in male plants. The PCR product of this primer was cloned and sequenced. The specific primers were synthesized for amplification of a 186 bp fragment in male date palm plants. These primers were validated in male and female date palm plants, wherein the designed SCAR marker was reported only in male plants and no amplification was observed in female plants. The developed SCAR marker was used with seedlings of date palm and proved very effective in identification of gender.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207878 | PMC |
| http://dx.doi.org/10.1155/2018/3035406 | DOI Listing |
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