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Setaria viridis as a Model Plant for Functional Genomic Studies in C4 Crops. | LitMetric

Setaria viridis as a Model Plant for Functional Genomic Studies in C4 Crops.

Methods Mol Biol

Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE), Brazilian Agricultural Research Corporation (EMBRAPA), Parque Estação Biológica, Brasília, DF, Brazil.

Published: June 2019

Setaria viridis is an emerging model for C4 species, and it is an important model to validate some genes for further C4 crop transformation, such as sugarcane, maize, and wheat. Here, we describe two protocols for stable transformation of S. viridis mediated by Agrobacterium tumefaciens with three different reporter genes and two selectable markers. Routine transformation efficiency reaching 29% was achieved using embryogenic callus in S. viridis (accession A10.1). Alternatively, we developed a transformation method by floral dip with 0.6% efficiency. The developed protocols could be useful for genetic and genomics studies of important food-feed-fiber-fuel C4 crops.

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Source
http://dx.doi.org/10.1007/978-1-4939-8778-8_4DOI Listing

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