The disaccharide β-d-mannopyranosyl-(1 → 2)-d-mannopyranose obtained by chemical cleavage and enzymatic dephosphorylation of biotechnologically available phosphomannan was transformed over six steps into a biotinylated probe suitable for assessment of carbohydrate specificity of antibodies induced by yeast cell wall preparations.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carres.2018.10.013DOI Listing

Publication Analysis

Top Keywords

biotinylated probe
8
assessment carbohydrate
8
carbohydrate specificity
8
specificity antibodies
8
synthesis biotinylated
4
probe biotechnologically
4
biotechnologically derived
4
derived β-d-mannopyranosyl-1 → 2-d-mannopyranose
4
β-d-mannopyranosyl-1 → 2-d-mannopyranose assessment
4
antibodies disaccharide
4

Similar Publications

Bioorthogonalized light-responsive click-and-uncage platform has enabled precise cell surface engineering and timed payload release, but most of such photoactivatable prodrugs have "always-on" photoactivity leading to the dark toxicity. On the other hand, the conditionally activatable photocage is limited to the application of fluorogenic probe/photosensitizer liberation. Herein, we devise a conditionally activatable theranostic platform based on the tetrazine (Tz)-boron-dipyrromethene (BODIPY) construct, in which tetrazine serves as a quencher motif to disable both the fluorescence and photoresponsivity of BODIPY.

View Article and Find Full Text PDF

Synthesis and preliminary evaluation of Tanshinone Mimic conjugates for mechanism of action studies.

Chembiochem

December 2024

CNR: Consiglio Nazionale delle Ricerche, Istituto di Scienze e Tecnologie Chimiche (SCITEC) 'Giulio Natta', ITALY.

Human antigen R (HuR) is an RNA binding protein (RBP) belonging to the ELAV (Embryonic Lethal Abnormal Vision) family, which stabilizes mRNAs and regulates the expression of multiple genes. Its altered expression or localization is related to pathological features such as cancer or inflammation. Dihydrotanshinone I (DHTS I) is a naturally occurring, tetracyclic ortho-quinone inhibitor of the HuR-mRNA interaction.

View Article and Find Full Text PDF

Effect of Linker Length on the Function of Biotinylated OSW-1 Probes.

Chembiochem

December 2024

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei-shi, Tokyo, 184-8588, JAPAN.

The biotinylated probes based on anticancer saponin OSW-1 with varied linker lengths were synthesized and their cell growth inhibitory activity and affinity pulldown efficiency were evaluated. All probes demonstrated comparable cytotoxicity to the parent natural product, highlighting that the linker moiety had a minimal impact on cell uptake or target engagement. In contrast, when evaluated against the known target proteins, OSBP and ORP4, the biotinylated probe 3 with PEG5 linker enabled most effective enrichment of target proteins in the affinity pulldown assay, suggesting that the cytotoxicity and pulldown efficiency did not correlate among the probes studied.

View Article and Find Full Text PDF

Electrochemistry-glucosemeter-smartphone integrated multi-mode biosensor for accurate detection of aflatoxin B1.

Anal Chim Acta

January 2025

College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, China. Electronic address:

Background: Aflatoxin B1 (AFB1) is a widely distributed toxic contaminant in food and poses a serious threat to public health. Therefore, an accurate, simple, cost-effective and on-site assay method is needed for sensitive detection of AFB1. Aptamer shows great potential in the construction of biosensor due to its high specificity and affinity.

View Article and Find Full Text PDF

Growth differentiation factor-15 (GDF-15) is a stress-responsive cytokine that increases in tissue injury and inflammatory states. The circulation level of GDF-15 is firmly correlated with cardiovascular diseases. Herein, we constructed a novel quantum dot-based fluorescent immunosensor for the sensitive detection of serum GDF-15.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!