During the process of cross-presentation, viral or tumor-derived antigens are presented to CD8 T cells by dependent CD8α/XCR1 classical dendritic cells (cDC1s). We designed a functional CRISPR screen for previously unknown regulators of cross-presentation, and identified the BEACH domain-containing protein WDFY4 as essential for cross-presentation of cell-associated antigens by cDC1s in mice. However, WDFY4 was not required for major histocompatibility complex class II presentation, nor for cross-presentation by monocyte-derived dendritic cells. In contrast to mice, mice displayed normal lymphoid and nonlymphoid cDC1 populations that produce interleukin-12 and protect against infection. However, similar to mice, mice failed to prime virus-specific CD8 T cells in vivo or induce tumor rejection, revealing a critical role for cross-presentation in antiviral and antitumor immunity.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6655551PMC
http://dx.doi.org/10.1126/science.aat5030DOI Listing

Publication Analysis

Top Keywords

wdfy4 required
8
cd8 cells
8
dendritic cells
8
mice mice
8
cross-presentation
6
mice
5
required cross-presentation
4
cross-presentation response
4
response viral
4
viral tumor
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!