Since its inception, Compact Disc (CD)-based centrifugal microfluidic technology has drawn a great deal of interest within research communities due to its potential use in biomedical applications. The technology has been referred to by different names, including compact-disc microfluidics, lab-on-a-disk, lab-on-a-CD and bio-disk. This paper critically reviews the state-of-the-art in CD-based centrifugal microfluidics devices and attempts to identify the challenges that, if solved, would enable their use in the extreme point of care. Sample actuation, manufacturing, reagent storage and implementation, target multiplexing, bio-particle detection, required hardware and system disposal, and sustainability are the topics of focus.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189906 | PMC |
| http://dx.doi.org/10.3390/mi7040052 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multiplex one-step direct asymmetric PCR of blood and dual-labelled probe-mediated melting curve for genotyping of MTHFR and MTRR polymorphisms.
RSC Adv
January 2025
Nanobiosensing and Microfluidic Point-of-Care Testing, Key Laboratory of Luzhou, Department of Clinical Laboratory, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University Luzhou Sichuan 646000 PR China
Accurate, rapid, and multiplex SNP analysis holds significant clinical value. However, the inevitable nucleic acid extraction, involving centrifugation, heating, and magnetic separation, is often time-consuming. In this study, direct blood PCR was combined with dual-labelled probe-mediated melting curves to identify SNPs corresponding to MTHFR (C677T, rs#1801133 and A1298C, rs#1801131) and MTRR (A66G, rs#1801394) in a single tube.
View Article and Find Full Text PDFAutomated Nanoliter Volume Assay Optimization on a Cost-Effective Microfluidic Disc.
Anal Chem
December 2024
Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, United States.
Optimizing multireagent assays often requires successive titration of individual components until the optimal combination of conditions is achieved. This process is time-consuming, laborious, and often expensive since parallelized experimentation requires bulk consumption of reagents. Microfluidics presents a solution through miniaturization of standard processes by reducing reaction volume, executing multiple parallel workflows, and enabling automation.
View Article and Find Full Text PDFA novel microfluidic device for human sperm separation based on rheotaxis.
Zygote
December 2024
Tissue Engineering Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
This study explores the efficacy of a novel microfluidic device in isolating rheotactic sperm and assesses their advantages compared with other motile sperm. Two microfluidic devices were used in this study: the microfluidic device we designed to separate sperm based on rheotaxis and a simple passive microfluidic device. We compared the results with the density gradient centrifugation technique.
View Article and Find Full Text PDFClinical validation and experiences of the microfluidics sperm selection device ZyMōt™ for standard IVF.
JBRA Assist Reprod
December 2024
Örebro University Department of Obstetrics and Gynecology Faculty of Medicine and Health Örebro Sweden Department of Obstetrics and Gynecology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Objective: Clinical validation of sperm selection device ZyMōt™ for standard IVF.
Methods: The pre-clinical validation of ZyMōt™ included several steps. First, split semen preparation compared density gradient centrifugation (DGC) to ZyMōt™ with primary outcome fraction and absolute number of progressive motile sperm.
A biomimetic sperm selection device for routine sperm selection.
Reprod Biomed Online
September 2024
University of Technology Sydney, Sydney, Australia; Institute for Biomedical Materials and Devices, University of Technology Sydney, Sydney, Australia. Electronic address:
Research Question: Can a biomimetic microfluidic sperm sorter isolate motile sperm while minimizing DNA damage in comparison with density gradient centrifugation (DGC)?
Design: This was a two-phase study of 61 men, consisting of a proof-of-concept study with 21 donated semen samples in a university research laboratory, followed by a diagnostic andrology study with 40 consenting patients who presented at a fertility clinic for semen diagnostics. Each sample was split to perform DGC and microfluidic sperm selection (one-step sperm selection with 15 min of incubation) side-by-side. Outcomes evaluated included concentration, progressive motility, and DNA fragmentation index (DFI) of raw semen, and sperm isolated using DGC and the microfluidic device.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!