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Expression Patterns, Genomic Conservation and Input Into Developmental Regulation of the GGDEF/EAL/HD-GYP Domain Proteins in . | LitMetric

AI Article Synopsis

  • The transition from vegetative growth to spore production in antibiotic-producing organisms is regulated by the signaling molecule c-di-GMP, which controls key developmental genes through the master regulator BldD.
  • A detailed study of all 10 proteins that influence c-di-GMP levels has shown that CdgB and RmdB are the most abundant within this group.
  • Genetic analysis revealed that the DGC CdgC is active, and disrupting certain DGCs enhances sporulation while disrupting PDEs delays development, with key proteins being conserved across multiple species in the genus.

Article Abstract

To proliferate, antibiotic-producing undergo a complex developmental transition from vegetative growth to the production of aerial hyphae and spores. This morphological switch is controlled by the signaling molecule cyclic bis-(3',5') di-guanosine-mono-phosphate (c-di-GMP) that binds to the master developmental regulator, BldD, leading to repression of key sporulation genes during vegetative growth. However, a systematical analysis of all the GGDEF/EAL/HD-GYP proteins that control c-di-GMP levels in is still lacking. Here, we have FLAG-tagged all 10 c-di-GMP turnover proteins in and characterized their expression patterns throughout the life cycle, revealing that the diguanylate cyclase (DGC) CdgB and the phosphodiesterase (PDE) RmdB are the most abundant GGDEF/EAL proteins. Moreover, we have deleted all the genes coding for c-di-GMP turnover enzymes individually and analyzed morphogenesis of the mutants in macrocolonies. We show that the composite GGDEF-EAL protein CdgC is an active DGC and that deletion of the DGCs and enhance sporulation whereas deletion of the PDEs and delay development in . By comparing the pan genome of 93 fully sequenced species we show that the DGCs CdgA, CdgB, and CdgC, and the PDE RmdB represent the most conserved c-di-GMP-signaling proteins in the genus .

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6205966PMC
http://dx.doi.org/10.3389/fmicb.2018.02524DOI Listing

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