Background: The porcine brain is gyrencephalic with similar gray and white matter composition and size more comparable to the human rather than the rodent brain; however, there is lack of information about neural progenitor cells derived from this model.
Results: Here, we isolated GFAP-positive porcine neural stem cells (NSCs) from the brain explant of a transgenic piglet, with expression of CreER under the control of the GFAP promoter (pGFAP-CreER). The isolated pGFAP-CreER NSCs showed self-renewal and expression of representative NSC markers such as Nestin and Sox2. Pharmacological inhibition studies revealed that Notch1 signaling is necessary to maintain NSC identity, whereas serum treatment induced cell differentiation into reactive astrocytes and neurons.
Conclusions: Collectively, these results indicate that GFAP promoter-driven porcine CreER NSCs would be a useful tool to study neurogenesis of the porcine adult central nervous system and furthers our understanding of its potential clinical application in the future. ᅟ.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222979 | PMC |
| http://dx.doi.org/10.1186/s12917-018-1660-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Neuropathology in intrauterine growth restricted newborn piglets is associated with glial activation and proinflammatory status in the brain.
J Neuroinflammation
January 2019
UQ Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Herston, QLD, 4029, Australia.
Background: The fetal brain is particularly vulnerable to intrauterine growth restriction (IUGR) conditions evidenced by neuronal and white matter abnormalities and altered neurodevelopment in the IUGR infant. To further our understanding of neurodevelopment in the newborn IUGR brain, clinically relevant models of IUGR are required. This information is critical for the design and implementation of successful therapeutic interventions to reduce aberrant brain development in the IUGR newborn.
View Article and Find Full Text PDFIsolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreER transgenic piglet.
BMC Vet Res
November 2018
Laboratory of Veterinary Embryology and Biotechnology, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, 1 Chungdae-ro, Seowon-gu, Cheongju, 28644, Republic of Korea.
Background: The porcine brain is gyrencephalic with similar gray and white matter composition and size more comparable to the human rather than the rodent brain; however, there is lack of information about neural progenitor cells derived from this model.
Results: Here, we isolated GFAP-positive porcine neural stem cells (NSCs) from the brain explant of a transgenic piglet, with expression of CreER under the control of the GFAP promoter (pGFAP-CreER). The isolated pGFAP-CreER NSCs showed self-renewal and expression of representative NSC markers such as Nestin and Sox2.
Mechanical disruption of the blood-brain barrier following experimental concussion.
Acta Neuropathol
May 2018
Department of Neurosurgery, Penn Center for Brain Injury and Repair, Perelman School of Medicine, University of Pennsylvania, 105 Hayden Hall, 3320 Smith Walk, Philadelphia, PA, 19104, USA.
Although concussion is now recognized as a major health issue, its non-lethal nature has limited characterization of the underlying pathophysiology. In particular, potential neuropathological changes have typically been inferred from non-invasive techniques or post-mortem examinations of severe traumatic brain injury (TBI). Here, we used a swine model of head rotational acceleration based on human concussion to examine blood-brain barrier (BBB) integrity after injury in association with diffuse axonal injury and glial responses.
View Article and Find Full Text PDFSpontaneous differentiation of porcine neural progenitors in vitro.
Cytotechnology
August 2011
Department of Spine Surgery, The First Hospital, Jilin University, Changchun, 130021, China.
The pig is the non-primate species that is immunologically closest to humans, and has been considered as an alternative source to human allografts for transplantation. In fact, there has been recent interest in identifying and culturing porcine neural progenitor cells (PNPCs) in vitro, but the long-term culturing has not yet been characterized. Here, we reported the spontaneous differentiation of PNPCs into neuronal and glial cells.
View Article and Find Full Text PDFCardiac arrest-induced regional blood-brain barrier breakdown, edema formation and brain pathology: a light and electron microscopic study on a new model for neurodegeneration and neuroprotection in porcine brain.
J Neural Transm (Vienna)
January 2011
Laboratory of Cerebrovascular Research, Department of Surgical Sciences, Anesthesiology and Intensive Care Medicine, University Hospital, 75185 Uppsala, Sweden.
Brief cardiac arrest and survival is often associated with marked neurological alterations related to cognitive and sensory motor functions. However, detail studies using selective vulnerability of brain after cardiac arrest in animal models are still lacking. We examined selective vulnerability of five brain regions in our well-established cardiac arrest model in pigs.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!