We propose a self-referential fast detection scheme for a frequency domain weak measurement system for the detection of enantiomeric impurities in chiral molecules. In a transmissive weak measurement system, the optical rotation (OR) is used to modify the pre-selected polarization state and the post-selection polarization state. We obtained the sum and difference of the optical rotations produced by the sample and the standard by rotating the quarter wave plate in the system. Then, we estimate the ratio of chiral molecules to enantiomeric impurities using the ratio of the central wavelength shifts caused by the addition and subtraction states described above. In this paper, our system has an optical resolution of 1.88 × 10°. At the same time, we completed the detection of the ratio of the two substances in the mixture of L-proline and D-proline in different proportions, which proved that our system can quickly detect the content of enantiomeric impurities in chiral molecules.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263648 | PMC |
| http://dx.doi.org/10.3390/s18113788 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparison of genotoxic impurities in extracted nicotine vs. synthetic nicotine.
Front Chem
October 2024
Porton Pharma Solutions, Chongqing, China.
Article Synopsis
- Nicotine, a chiral alkaloid mainly sourced from tobacco, is used as the addictive agent in smoking products, while synthetic nicotine has gained popularity for being cheaper and possibly purer, lacking impurities from plant extraction.
- * The U.S. FDA has established new regulations on synthetic nicotine quality control, but comprehensive research comparing its impurity profile with tobacco-extracted nicotine is still limited.
- * In a study comparing 13 lots of synthetic nicotine and 14 lots from tobacco, similar total impurity levels were found (0.1%), but synthetic nicotine did not contain certain impurities like cotinine and nornicotine, and exhibited high enantiomeric purity.
Development of a certified reference material for D-phenylalanine with evaluation of enantiomeric purity.
Anal Bioanal Chem
September 2024
Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China.
D-Phenylalanine (D-Phe) is a small chiral organic molecule that is both an important pharmaceutical intermediate and used as a calibrator for quantifying amino acids in liquid chromatography-circular dichroism. We have developed a process for a national certified reference material (CRM) for D-Phe following ISO 17034:2016. The identity of D-Phe was confirmed using mass spectrometry (MS) and nuclear magnetic resonance (NMR), infrared, and ultraviolet (UV) spectroscopy.
View Article and Find Full Text PDFTandem Mass Spectrometry Approaches for Differentiation and Quantification Pidotimod and Its Three Isomers in the Gas Phase.
Chirality
August 2024
College of New Materials and Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing, China.
Pidotimod is a chiral drug that possesses two chiral centers, resulting in three isomeric impurities (analytes, A). This study employs electrospray ionization ion trap mass spectrometry (ESI-MS) through collision-induced dissociation (CID) to investigate the chiral recognition of pidotimod and its three isomers to eliminate chromatographic separation. Three approaches were explored: (1) Protonated molecules in CID exhibited discriminative potential for diastereomers, with the ability to distinguish between S,S and R,R configurations, albeit with an R value of ~1.
View Article and Find Full Text PDFFast, sensitive LC-MS resolution of -hydroxy acid biomarkers via SPP-teicoplanin and an alternative UV detection approach.
Anal Bioanal Chem
May 2024
Department of Chemistry and Biochemistry, University of Texas at Arlington, 700 Planetarium Place, Arlington, TX, 76019, USA.
Enantioseparation of -hydroxy acids is essential since specific enantiomers of these compounds can be used as disease biomarkers for diagnosis and prognosis of cancer, brain diseases, kidney diseases, diabetes, etc., as well as in the food industry to ensure quality. HPLC methods were developed for the enantioselective separation of 11 -hydroxy acids using a superficially porous particle-based teicoplanin (TeicoShell) chiral stationary phase.
View Article and Find Full Text PDFSimultaneous Determination of Enantiomeric Purity and Organic Impurities of Dexketoprofen Using Reversed-Phase Liquid Chromatography-Enhancing Enantioselectivity through Hysteretic Behavior and Temperature-Dependent Enantiomer Elution Order Reversal on Polysaccharide Chiral Stationary Phases.
Int J Mol Sci
February 2024
Department of Pharmaceutical Chemistry, Semmelweis University, Hogyes 9, H-1092 Budapest, Hungary.
A reversed-phase high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of the potential impurities of dexketoprofen, including the distomer R-ketoprofen. After screening the separation capability of four polysaccharide columns (Lux Amylose-1, Lux Amylose-2, Lux Cellulose-1 and Lux Cellulose-2) in polar organic and in reversed-phase modes, appropriate enantioseparation was observed only on the Lux Amylose-2 column in an acidified acetonitrile/water mixture. A detailed investigation of the mobile phase composition and temperature for enantio- and chemoselectivity showed many unexpected observations.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!