AI Article Synopsis
- Next-generation sequencing helps identify genetic variations in rice that affect grain quality, particularly in starch content.
- Researchers can focus on specific genes related to starch or analyze the entire rice genome, depending on what they already know about the traits.
- Techniques like amplicon sequencing and whole-genome re-sequencing are used to uncover the genetic factors influencing starch quality in rice.
Article Abstract
Next-generation sequencing can identify differences in the rice genome that explain the genetic basis of grain quality variation. Differences in rice grain quality are mainly associated with differences in the major component of the grain, starch. Association of rice quality variation with rice genome variation can be conducted at the gene or whole-genome level. Re-sequencing of specific genes or whole genomes can be used depending on the extent to which candidate genes for the traits of interest are known. Amplicon sequencing of genes involved in starch metabolism can help in targeted discovery of the molecular genetic basis of differences in starch related quality attributes. Whole-genome re-sequencing can complement this, when the genetic basis of the trait is expected to be outside the coding region of starch metabolism genes. These approaches have been used successfully to understand the rice genome at specific loci and over the whole genome.
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| http://dx.doi.org/10.1007/978-1-4939-8914-0_12 | DOI Listing |
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