In 2015, was separated from on the basis of phylogenomic and phylogenetic studies, and urease activity was reported as a phenotypic property able to differentiate between the two species. Subsequently, we have found that the urease activity of is strain-specific, and does not reliably discriminate between species, as strains having the same urease gene cluster were identified in and , the closest relatives of . We developed a multilocus sequence typing scheme using eight housekeeping genes, , and to clearly identify from closely related species and to find a molecular marker for the rapid identification of . The scheme differentiated 33 strains from 90 strains formerly identified as . Among the eight housekeeping genes, possesses appropriate polymorphic sites for the design of a -specific PCR primer set. The primer set designed in this study perfectly separated from and .
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