MicroRNAs (miRNAs), small non-coding RNAs, have emerged as important, epigenetic regulators of endothelial function. Metabolic disturbances such as diabetes alter miRNA expression. In adults, the miRNA transcriptome as well as endothelial function differ between the sexes. Here, we hypothesized that metabolic disturbances associated with gestational diabetes (GDM) alter miRNA signatures in feto-placental endothelial cells (fpEC), dependent on fetal sex. We isolated human primary fpEC after normal and GDM-complicated pregnancies with male and female neonates and screened for differential miRNA expression using next-generation miRNA sequencing. To test for miRNAs commonly regulated in fpEC of female and male progeny, data were stratified for fetal sex and maternal body mass index (BMI). Analyses were also performed separately for female and male fpEC, again accounting for maternal BMI as covariate. Potential biological pathways regulated by the altered set of miRNAs were determined using mirPath software. Maternal GDM altered 26 miRNA signatures when male and female fpEC were analyzed together. Separate analysis of male versus female fpEC revealed 22 GDM affected miRNAs in the females and only 4 in the males, without overlap. Biological functions potentially modulated by the affected miRNAs related to 'Protein Processing in Endoplasmic Reticulum' and 'Proteoglycans in Cancer'. Maternal GDM alters miRNA signatures in fpEC, and biological functions affected by these miRNAs relate to well-known adverse functional consequences of diabetes on endothelium. GDM effects were highly dependent on fetal sex with miRNA signatures in female fpEC being more susceptible to metabolic derangements of GDM than miRNAs in male fpEC.
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http://dx.doi.org/10.1042/CS20180825 | DOI Listing |
Nutrients
January 2025
Department of Maternal-Fetal Biology, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan.
Background/objectives: The DNA methylation of neonatal cord blood can be used to accurately estimate gestational age. This is known as epigenetic gestational age. The greater the difference between epigenetic and chronological gestational age, the greater the association with an inappropriate perinatal fetal environment and development.
View Article and Find Full Text PDFBiology (Basel)
January 2025
Hunan Engineering Research Center of Ecological Environment Intelligent Monitoring and Disaster Prevention and Mitigation Technology in Dongting Lake Region, College of Information and Electronic Engineering, Hunan City University, Yiyang 413000, China.
Plant sterilants are used to control rodent populations due to their minimal environmental risk and other ethical considerations. However, their practical utilization is unsatisfactory due to high costs and processing difficulties. is a plant material that has shown the potential to inhibit the reproduction of , a species that causes serious damage to crops in the Dongting Lake region in China.
View Article and Find Full Text PDFBMC Pediatr
January 2025
Institute of Child Health, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Background: Currently, macrosomia contributes to maternal and neonatal morbidity and mortality in low-and middle-income countries because of changes in maternal lifestyle. Reliable data are needed for its prevention, early detection, and management. This study assessed the associations between sociodemographic, anthropometric, maternal lifestyle, perinatal outcomes, and macrosomia in Southwest Nigeria.
View Article and Find Full Text PDFSci Rep
January 2025
Institute of Health Sciences, Collegium Salutis Humanae, University of Opole, Opole, 45-060, Poland.
Just as overweight and obesity may impair immunity, excessive body weight-related parameters of women in the pre-conception period and during pregnancy are possible detrimental factors for fetal programming of the immune system in their offspring. We investigated the relationship of pre-pregnancy body mass index (pBMI) and gestational weight gain (GWG) of mothers with the placental transport rate (PTR) of IgG antibodies and antineutrophil cytoplasmatic antibodies against lactoferrin (Lf-ANCA) and their concentration in umbilical cord blood serum (UCS), verifying the sex-specificity of this relationship. The examined group of this cross-sectional pilot study consisted of 101 pregnant women and their healthy CS-delivered newborn children.
View Article and Find Full Text PDFAlcohol
January 2025
Department of Veterinary Physiology & Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA, 77843. Electronic address:
Emerging research reveals that alcohol use by fathers before conception can affect the growth and development of their offspring. Here, we used a C57BL/6J mouse model to study the effects of alcohol exposure on the behavior of the first-generation (F1) offspring, comparing the impacts of alcohol exposure by mothers, fathers, and both parents. Our goal was to determine how alcohol exposure by each parent or both parents influences the behavior of the offspring.
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