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A study developed EST-SSR markers for a plant in the Cyperaceae family to explore its evolutionary background, particularly in Japan's solfatara fields.
Using RNA-Seq technology, 20 markers were created and analyzed for genetic variation in this plant and related species, revealing low genetic polymorphism due to population dynamics.
The findings will aid future research on genetic diversity and evolutionary history in this plant and its relatives.
Article Abstract
Premise Of The Study: Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed for (Cyperaceae) to investigate the evolutionary history of this plant that is endemic to solfatara fields in northern Japan.
Methods And Results: Using RNA-Seq data generated by the Illumina HiSeq 2000, 20 EST-SSR markers were developed. Polymorphisms were assessed in and the closely related species and . In , many loci were monomorphic within populations; the average number of alleles ranged from one to five, and levels of expected heterozygosity ranged from 0.000 to 0.580, while all markers were polymorphic in a population of . This indicates that low genetic polymorphism of is likely due to the species' population dynamics, rather than to null alleles at the developed markers.
Conclusions: These markers will be used to assess genetic diversity and structure and to investigate evolutionary history in future studies of and related species.
