Reference Values for WBC Differential by Hematoflow Analysis.

Objectives: WBC differentials performed using flow cytometry with monoclonal antibodies have been developed in the last decade and are nowadays integrated into the routine workflow of some laboratories. Definition of reference values for each population is required in order to achieve an automatic validation of the results by laboratory software.

Methods: We analyzed 584 samples from three hospitals using the Hematoflow solution to define the reference values.

Results: Reference values are presented for five groups according to age (0-5, 6-11, 12-19, 20-69, and >69 years).

Conclusions: These normal values will be helpful in the definition of relevant threshold for the automatic validation of samples analyzed by flow cytometry and the flagging of pathologic samples.