AI Article Synopsis
- GABA increases 36Cl- influx in rat cerebellar granule cell cultures by two-fold when 10(-5) M GABA is added, peaking at about 20 seconds.
- Bicuculline, a GABAA receptor antagonist, reduces this chloride influx when the receptors are progressively occupied.
- The findings indicate that these primary cultures of rat cerebellar granule cells are a reliable model for studying GABA-activated chloride transport.
Article Abstract
gamma-Aminobutyric acid (GABA)-mediated and bicuculline-sensitive 36Cl- influx and bicuculline-sensitive [3H] GABA binding were demonstrated in cultures of rat cerebellar granule cells. The addition of 10(-5) M GABA produced a two-fold increase in 36Cl-influx over the basal level and the maximal increase was observed after approximately 20 sec. Progressive occupation of GABAA receptor by [3H]-(1S-9R)-bicuculline methiodide decreased 36Cl- influx activated by 10 microM GABA. The above results suggest that primary cultures of rat cerebellar granule cells provide a new and reliable model for studying the GABA activated chloride fluxes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0024-3205(87)90148-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Integrating major ion geochemistry, stable isotopes (O, H) and radioactive isotopes (Rn, C, Cl, H) to understand the interaction between catchment waters and an intermittent river.
Sci Total Environ
January 2024
Department of Nuclear Physics and Accelerator Applications, Research School of Physics, The Australian National University, Canberra, ACT 2601, Australia.
Determining the locations and sources of baseflow and the transit times of water is important for understanding catchment behaviour and functioning. Major ion geochemistry, stable isotopes (O and H), and radioisotopes (Rn, H, C, and Cl) were used to investigate the sources and transit times of water in the upper catchment of the intermittent Avoca River in southeast Australia. Rn activities and Cl concentrations implied the presence of baseflow inputs and the distribution was mainly controlled by local topography.
View Article and Find Full Text PDFEffects of sodium chloride exposure on ion regulation in larvae (glochidia) of the freshwater mussel Lampsilis fasciola.
Ecotoxicol Environ Saf
December 2015
Aquatic Contaminants Research Division, Water and Science Technology Directorate, Environment Canada, Burlington, ON, Canada L7R-4A6.
The salinization of freshwater can have negative effects on ecosystem health, with heightened effects in salt-sensitive biota such as glochidia, the larvae of freshwater mussels. However, the toxicological mechanism underlying this sensitivity is unknown. Therefore, Lampsilis fasciola glochidia were exposed to NaCl (nominally 0.
View Article and Find Full Text PDFMolecular cloning and functional characterization of zebrafish Slc4a3/Ae3 anion exchanger.
Pflugers Arch
August 2014
Renal Division and Molecular and Vascular Medicine Division, Beth Israel Deaconess Medical Center; Department of Medicine, Harvard Medical School, 99 Brookline Ave. RN-380 F, Boston, MA, 02215, USA.
The zebrafish genome encodes two slc4a1 genes, one expressed in erythroid tissues and the other in the HR (H(+)-ATPase-rich) type of embryonic skin ionocytes, and two slc4a2 genes, one in proximal pronephric duct and the other in several extrarenal tissues of the embryo. We now report cDNA cloning and functional characterization of zebrafish slc4a3/ae3 gene products. The single ae3 gene on chromosome 9 generates at least two low-abundance ae3 transcripts differing only in their 5'-untranslated regions and encoding a single definitive Ae3 polypeptide of 1170 amino acids.
View Article and Find Full Text PDFExcitation-induced exchange of Na+, K+, and Cl- in rat EDL muscle in vitro and in vivo: physiology and pathophysiology.
J Gen Physiol
February 2013
Department of Biomedicine, Aarhus University, DK-8000 Aarhus C, Denmark.
In skeletal muscle, excitation leads to increased [Na(+)](i), loss of K(+), increased [K(+)](o), depolarization, and Cl(-) influx. This study quantifies these changes in rat extensor digitorum longus (EDL) muscles in vitro and in vivo using flame photometric determination of Na(+) and K(+) and (36)Cl as a tracer for Cl(-). In vitro, 5-Hz stimulation for 300 s increased intracellular Na(+) content by 4.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!