AI Article Synopsis
- Pinnatane A, derived from bark, exhibits strong anti-cancer properties by causing cell death in liver cancer cell lines.
- The study utilized various assays, such as the MTT assay and flow cytometry, to analyze its selective toxicity and the mechanisms behind cell cycle arrest and apoptosis.
- Results indicated significant dose-dependent cytotoxicity in cancer cells with minimal impact on normal cells, suggesting its potential as a chemotherapeutic agent for liver cancer.
Article Abstract
Background: Pinnatane A from the bark of was investigated for its anti-cancer properties by analyzing the cytotoxic activities and cell cycle arrest mechanism induced in two different liver cancer cell lines.
Methods: A 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to analyze the pinnatane A selectivity in inducing cell death in cancer and normal cells. Various biological assays were carried out to analyze the anti-cancer properties of pinnatane A, such as a live/dead assay for cell death microscopic visualization, cell cycle analysis using propidium iodide (PI) to identify the cell cycle arrest phase, annexin V-fluorescein isothiocyanate (annexin V-FITC)/PI flow cytometry assay to measure percentage of cell populations at different stages of apoptosis and necrosis, and DNA fragmentation assay to verify the late stage of apoptosis.
Results: The MTT assay identified pinnatane A prominent dose- and time-dependent cytotoxicity effects in Hep3B and HepG2 cells, with minimal effect on normal cells. The live/dead assay showed significant cell death, while cell cycle analysis showed arrest at the G₀/G₁ phase in both cell lines. Annexin V-FITC/PI flow cytometry and DNA fragmentation assays identified apoptotic cell death in Hep3B and necrotic cell death in HepG2 cell lines.
Conclusions: Pinnatane A has the potential for further development as a chemotherapeutic agent prominently against human liver cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278294 | PMC |
| http://dx.doi.org/10.3390/molecules23112733 | DOI Listing |
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